Genetically Modified Organism Research Center

Transgenic Silkworm Research Unit


2013~Latest・2010~20122007~2009

  1. Tsubota T, Tomita S, Uchino K, Kimoto M, Takiya S, Kajiwara H, Yamazaki T, Sezutsu H (2016) A Hox gene Antennapedia regulates expression of multiple major silk protein genes in the silkworm Bombyx mori The Journal of Biological Chemistry doi: 10.1074/jbc.M115.699819
  2. Nakade S, Tsubota T, Sakane Y, Kume S, Sakamoto N, Obara M, Daimon T, Sezutsu H, Yamamoto T, Sakuma T and Suzuki K (2014) Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9 Nature Communications DOI:10.1038/ncomms6560
  3. Kuwana Y, Sezutsu H, Nakajima K, Tamada Y, Kojima K (2014) High-toughness silk produced by a transgenic silkworm expressing spider (Araneus ventricosus) dragline silk protein PLoS One DOI: 10.1371/journal.pone.0105325
  4. Tsubota T, Uchino K, Suzuki T.K, Tanaka H, Kayukawa T, Shinoda T, Sezutsu H (2014) Identification of a novel strong and ubiquitous promoter/enhancer in the silkworm Bombyx mori G3: Genes, Genomes, Genetics 4(7):1347-1357 (DOI: 10.1534/g3.114.011643)
  5. Tatematsu KI, Uchino K, Sezutsu H, Tamura T (2014) Effect of ATG initiation codon context motifs on the efficiency of translation of mRNA derived from exogenous genes in the transgenic silkworm, Bombyx mori SpringerPlus 3:136
  6. Mun S, Noh MY, Osanai-Futahashi M, Muthukrishnan S, Kramer KJ, Arakane Y (2014) A Major Facilitator Superfamily protein encoded by TcMucK gene is not required for cuticle pigmentation, growth and development in Tribolium castaneum Insect Biochemistry and Molecular Biology 49:43-8
  7. Kayukawa T, Murata M, Kobayashi I, Muramatsu D, Okada C, Uchino K, Sezutsu H, Kiuchi M, Tamura T, Hiruma K, Ishikawa Y (2014) Hormonal regulation and developmental role of Kruppel homolog 1, a repressor of metamorphosis, in the silkworm Bombyx mori Developmental Biology 388(1):48-56
  8. Kimoto M, Tsubota T, Uchino K, Sezutsu H, Takiya S (2014) Hox transcription factor Antp regulates sericin-1 gene expression in the terminal differentiated silk gland of Bombyx mori Developmental Biology386(1):64-71
  9. Tsubota T, Uchino K, Kamimura M, Ishikawa M, Hamamoto H, Sekimizu K, Sezutsu H (2014) Establishment of transgenic silkworms expressing GAL4 specifically in hemocytes Insect Molecular Biology 23(2):165-74
  10. Takasu Y, Tamura T, Sajwan S, Kobayashi I, Zurovec M (2014) The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitfly MethodsS1046-2023(14)00044-9
  11. Mon H, Li Z, Kobayashi I, TomitaS, Lee JM, Sezutsu H, Tamura T, Kusakabe T (2013) Arrest of cell cycle progression by soaking RNAi in cultured silkworm BmN4-SID1 cells Journal of Insect Science 2013. 13.155
  12. Iizuka T, Sezutsu H, Tatematsu KI, Kobayashi I, Yonemura N, Uchino K, Nakajima K, Kojima K, Takabayashi C, Machii H, Yamada K, Kurihara H, Asakura T, Nakazawa Y, Miyawaki A, Karasawa S, Kobayashi H, Yamaguchi J, Kuwabara N, Nakamura T, Yoshii K, Tamura T (2013) Colored fluorescent silk made by transgenic silkworms Advanced Functional Materials 23(42):5232-5239
  13. Yonemura N, Tamura T, Uchino K, Kobayashi I, Tatematsu KI, Iizuka T, Tsubota T, Sezutsu H (2013) PhiC31 integrase-mediated, site-specific integration of transgenes in the silkworm, Bombyx mori (Lepidoptera: Bombycidae)Applied Entomology and Zoology 48(3):265-273
  14. Yukuhiro K, Sezutsu H, Tamura T, Kosegawa E, Iwata K, Ajimura M, Gu S-H, Wang M, Xia Q, Mita K, Kiuchi M (2013) Little gene flow between domestic silkmoth Bombyx mori and its wild relative Bombyx mandarina in Japan, and possible artificial selection on the CAD gene of B. mori Genes & Genetic Systems 87(5):331-340
  15. Sezutsu H, Le Goff G, Feyereisen R (2013) Origins of P450 diversity Philosophical Transactions B 369: 20120428
  16. Ohno K, Sawada J, Takiya S, Kimoto M, Matsumoto A, Tsubota T, Uchino K, Hui C, Sezutsu H, Handa H, Suzuki Y (2013) Silk gland factor-2 (SGF-2) involved in fibroin gene transcription consists of LIM-homeodomain, LIM-interacting, and single-stranded DNA-binding proteins Journal of Biological Chemistry 288(44):31581-91
  17. Tabuchi M, Sakurai T, Mitsuno H, Namiki S, Minegishi R, Shiotsuki T, Uchino K, Sezutsu H, Tamura T, Haupt SS, Nakatani K, Kanzaki R (2013) Pheromone responsiveness threshold depends on temporal integration by antennal lobe projection neurons Proceedings of the National Academy of Sciences of the United States of America 110(38):15455-15460
  18. Yamamoto DS, Yokomine T, Sumitani M, Yagi K, Matsuoka H, Yoshida S (2013) Visualization and live imaging analysis of a mosquito saliva protein in host animal skin using a transgenic mosquito with a secreted luciferase reporter system Insect Mol Biology 22(6):685-693
  19. Suetsugu Y, Futahashi R, Kanamori H, Kadono-Okuda K, Sasanuma S, Narukawa J, Ajimura M, Jouraku A, Namiki N, Shimomura M, Sezutsu H, Osanai-Futahashi M, Suzuki M.G, Daimon T, Shinoda T, Taniai K, Asaoka K, Niwa R, Kawaoka S, Katsuma S, Tamura T, Noda H, Kasahara M, Sugano S, Suzuki Y, Fujiwara H, Kataoka H, Arunkumar K.P, Tomar A, Nagaraju J, Goldsmith M.R, Feng Q, Xia Q, Yamamoto K, Shimada T, Mita K (2013) Large scale full-Length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori G3: Genes, Genomes, Genetics 3(9):1481-1492
  20. Takasu Y, Sajwan S, Daimon T, Osanai-Futahashi M, Uchino K, Sezutsu H, Tamura T, Zurovec M (2013) Efficient TALEN construction for Bombyx gene targeting PLoS One 8(9):e73458
  21. Sajwan S, Takasu Y, Tamura T, Uchino K, Sezutsu H, Zurovec M (2013) Efficient disruption of endogenous Bombyx gene by TAL effector nucleases Insect Biochemistry and Molecular Biology 43(1):17-23
  22. Tatsuke T, Lee JM, IIyama K, Sezutsu H, Uchino K, Kusakabe T (2013) Tightly controlled Tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells Archives of Insect Biochemistry & Physiology 82(4):173-182