[Advances in Technology]
[Background]
Fig.1. Scheme of high-throughput measurement Fof isgeed. s1hape using SmartGrain |
Fig.2. SmartGrain automatically identifies seeds within an image and measures the seed shape profiles. (A) Image of grains on scanner. Red arrow indicates a pedicel. (B) SmartGrain image of grains. (C) Grain shape parameters: AS, within red line; PL, red line; CS, from red line; L, yellow line; W, green line; IS, white circle; CG, red circle. (D) Algorithm for image analysis and measurement of seed shape profiles. |
Fig.3. Seed L and W of SL635, SL636, and SL637 CSSLs with a cv Nipponbare segment of chromosome 11 in Koshihikari background. (A) Graphical genotypes of chromosome 11 in the CSSLs. Gray, homozygous for Koshihikari; white, homozygous for Nipponbare. (B) Seeds of the three CSSLs and Koshihikari; bar = 5 mm. L (C) and 1,000-grain weight (D; dehulled) of parents and CSSLs. Asterisks indicate significant differences between Koshihikari and the CSSLs: *P < 0.05, **P < 0.01; ns, not significant. L was measured in five plants per line, and 1,000-seed weight was measured independently three times in bulked grains of each line. |
[Reference]