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Fujimoto, T., Mizukubo, T., Abe, H., Seo, S. Sclareol induces plant resistance to root-knot nematode partially through ethylene-dependent enhancement of lignin accumulation. Mol. Plant-Microbe Interact. in press
DOI: http://dx.doi.org/10.1094/MPMI-10-14-0320-R

Hasegawa, M., Mitsuhara, I., Seo, S., Okada, K., Yamane, H., Iwai, T., Ohashi, Y. (2014) Analysis on blast fungus-responsive characters of a flavonoid phytoalexin sakuranetin; accumulation in infected rice leaves, antifungal activity and detoxification by fungus. Molecules 19: 11404-11418
DOI: http://dx.doi.org/10.1104/pp.113.222802


Abe, H., Tateishi, K., Seo, S., Kugimiya, S., Yokota-Hirai, M., Sawada, Y., Murata, Y., Yara, K., Shimoda, T., Kobayashi, M. (2013) Disarming the jasmonate-dependent plant defense makes nonhost Arabidopsis plants accessible to the American serpentine leafminer. Plant Physiol. 163: 1242-1253
DOI: 10.3390/molecules190811404

Katous, S., Asakura, N., Kojima, T., Mitsuhara, I., Seo, S. (2013) Transcriptome analysis of WIPK/SIPK-suppressed plants reveals induction by wounding of disease resistance-related genes prior to the accumulation of salicylic acid. Plant Cell Physiol. 54: 1005-1015
DOI: 10.1093/pcp/pct055

Kawazu, K., Mochizuki, A., Sugeno, W., Seo, S., Mitsuhara, I. (2013) Differences in the susceptibility of five herbivore species and developmental stages to tomato resistance induced by methyl jasmonate treatment. Arthropod-Plant Interactions 7: 415-422
DOI: 10.1007/s11829-013-9257-00

Oka, K., Kobayashi, M., Mitsuhara, I., Seo, S. (2013) Jasmonic acid negatively regulates resistance to Tobacco mosaic virus in tobacco. Plant Cell Physiol. 54: 1999-2010
DOI: 10.1093/pcp/pct137

Oka, K., Amano, Y., Katou, S., Seo, S., Kawazu, K., Mochizuki, A., Kuchitsu, K., Mitsuhara, I. (2013) Tobacco MAP kinase phosphatase (NtMKP1) negatively regulates wound response and induced resistance against necrotrophic pathogens and lepidopteran herbivores. Mol. Plant-Microbe Interact. 26: 668-675
DOI: http://dx.doi.org/10.1094/MPMI-11-12-0272-R

Suzuki, N., Imai, L.F., Kato, Y., Nagata, K., Ohashi, Y., Kuchitsu, K., Tanokura, M., Sakamoto, A., Nara, M., Nakano, M., Yonezawa, N. (2013) Coordination structures of Mg2+ and Ca2+ in three types of tobacco calmodulins in solution: Fourier-transform infrared spectroscopic studies of side-chain COO- groups. Biopolymers 99: 472-483
DOI: 10.1002/bip.22203

Tamaoki, D., Seo, S., Yamada, S., Kano, A., Miyamoto, A., Shishido, H., Miyoshi, S., Taniguchi, S., Akimitsu, K., Gomi, K. (2013) Jasmonic acid and salicylic acid activate a common defense system in rice. Plant Signaling & Behavior 8: e24260


Abe, H., Tomitaka, Y., Shimoda, T., Seo, S., Sakurai, T., Kugimiya, S., Tsuda, S., and Kobayashi, M. (2012) Antagonistic plant defense system regulated by phytohormones assists interactions among vector insect, thrips and a tospovirus. Plant Cell Physiol. 53:204-212
DOI: 10.1093/pcp/pcr173

Asano, T., Hayashi, N., Kobayashi, M., Aoki, N., Miyao, A., Mitsuhara, I., Ichikawa, H., Komatsu, S., Hirochika, H., Kikuchi, S., and Ohsugi, R. (2012) A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance. Plant J. 69: 26-36.
DOI: 10.1111/j.1365-313X.2011.04766.x

Imai, T., Ohashi, Y., Mitsuhara, I., Seo, S., Toshima, H., and Hasegawa, M. (2012) Identification of a degradation intermediate of the momilactone a rice phytoalexin by the rice blast fungus. Biosci. Biotechnol. Biochem. 76:414-416.
DOI: 10.1271/bbb.110756

Kawazu, K., Mochizuki, A., Sato, Y., Sugeno, W., Murata, M., Seo, S., Mitsuhara, I. (2012) Different expression profiles of jasmonic acid and salicylic acid inducible genes in the tomato plant against herbivores with various feeding modes. Arthropod-Plant Interactions 6:221-230.
DOI: 10.1007/s11829-011-9174-z

Seo, S., Gomi, K., Kaku, H., Abe, H., Seto, H., Nakaho, K., Nakatsu, S., Neya, M., Kobayashi, M., Ichinose, Y., Mitsuhara, I., and Ohashi, Y. (2012) Identification of natural diterpenes that inhibit bacterial wilt disease in tobacco, tomato and Arabidopsis. Plant Cell Physiol.
DOI: 10.1093/pcp/pcs085 in press


Kobayashi, M.*, Yamamoto-Katou, A.*, Katou, S.*, Hirai, K., Meshi, T., Ohashi, Y., and Mitsuhara, I. (2011) Identification of an amino acid residue required for differential recognition of a viral movement protein by the Tomato mosaic virus resistance gene Tm-22. J. Plant Physiol. 168: 1142-1145. (*co-first authors)
DOI: 10.1016/j.jplph.2011.01.002

Seo, S.*, Mitsuhara, I.*, Feng, J., Iwai, T., Hasegawa, M., and Ohashi, Y. (2011) Cyanide, a co-product of plant hormone ethylene biosynthesis, contributes to the resistance of rice to blast fungus. Plant Physiol. 155: 502-514.  (*co-first authors)
DOI: 10.1104/pp.110.162412


Hasegawa, M.*, Mitsuhara, I.*, Seo, S.*, Imai, T., Koga, J., Okada, K., Yamane, H., and Ohashi, Y. (2010) Phytoalexin accumulation in the interaction between rice and the blast fungus. Mol. Plant-Microbe Interact. 23: 1000-1011. (*co-first authors)
DOI: 10.1094/MPMI-23-8-1000

Iki, T., Yoshikawa, M., Nishikiori, M., Jaudal, M.C., Matsumoto-Yokoyama, E., Mitsuhara, I., Meshi, T., and Ishikawa, M. (2010) In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90. Mol. Cell 39: 282-291.
DOI: 10.1016/j.molcel.2010.05.014

Kobayashi, M.*, Seo, S.*, Hirai, K., Yamamoto-Katou, A., Katou, S., Seto, H., Meshi, T., Mitsuhara, I., and Ohashi, Y. (2010) Silencing of WIPK and SIPK mitogen-activated protein kinases reduces Tobacco mosaic virus accumulation but permits systemic viral spread in tobacco possessing the N resistance gene. Mol. Plant-Microbe Interact. 23:1032-1041. (*co-first authors)
DOI: 10.1094/MPMI-23-8-1032

Kobayashi, M., Ishihama, N., Yoshioka, H., Takabatake, R., Tsuda, S., Seo, S., Ohashi, Y., and Mitsuhara, I. (2010) Analyses of the cis-regulatory regions responsible for the transcriptional activation of the N resistance gene by Tobacco mosaic virus. J. Phytopathol. 158: 826-828.


Abe, H., Shimoda, T., Ohnishi, J., Kugimiya, S., Narusaka, M., Seo, S., Narusaka, Y., Tsuda, S., and Kobayashi, M. (2009) Jasmonate-dependent plant defense restricts thrips performance and preference. BMC Plant Biol.  9: 97.

Gomi, K., Satoh, M., Ozawa, R., Shinonaga, Y., Sanada, S., Sasaki, K., Matsumura, M., Ohashi, Y., Kanno, H., Akimitsu, K., and Takabayashi, J. (2009) Role of hydroperoxide lyase in white-backed planthopper (Sogatella furcifera Horvath)-induced resistance to bacterial blight in rice, Oryza sativa L. Plant J. 61: 46-57.
DOI: 10.1111/j.1365-313X.2009.04031.x

Hiraga, S.*, Sasaki, K.*, Hibi, T.*, Yoshida, H., Uchida, E., Kosugi, S., Kato, T., Mie, T., Ito, H., Katou, S., Seo, S., Matsui, H., Ohashi, Y., and Mitsuhara, I. (2009) Involvement of two rice ETHYLENE INSENSITIVE3-LIKE genes in wound signaling. Mol. Gen. Genomics 282: 517-529. (*co-first authors)


Abe, H., Ohnishi, J., Narusaka, M., Seo, S., Narusaka, Y., Tsuda, S., and Kobayashi, M. (2008) Function of jasmonate in response and tolerance of Arabidopsis to thrip feeding. Plant Cell Physiol. 49: 68-80.
DOI: 10.1093/pcp/pcm168

Abe, H, Ohnishi, J., Narusaka, M., Seo, S., Narusaka, Y., Tsuda, S., and Kobayashi, M. (2008) Arabidopsis: Thrips system for analysis of plant response to insect feeding. Plant Signaling & Behavior  3: 446-447.
DOI: 10.4161/psb.3.7.5556

Hase, S., Takahashi, S., Takenaka, S., Nakaho, K., Arie, T., Seo, S., Ohashi, Y., and Takahashi, H. (2008) Involvement of jasmonic acid signaling in bacterial wilt disease resistance induced by biocontrol agent Pythium oligandrum in tomato. Plant Pathol. 57: 870-876.
DOI: 10.1111/j.1365-3059.2008.01858.x

Ishihara, T., Sekine, K.-T., Hase, S., Kanayama, Y., Seo, S., Ohashi, Y., Kusano, T., Shibata, D., Shah, J., and Takahashi, H. (2008) Overexpression of the Arabidopsis thaliana EDS5 gene enhances resistance to viruses. Plant Biology 10: 451-461.
DOI: 10.1111/j.1438-8677.2008.00050.x

Narusaka, M., Kawai, K., Izawa, N., Seki, M., Shinozaki, K., Seo, S., Kobayashi, M., Shiraishi, T., and Narusaka, Y. (2008) Gene coding for sigA-binding peotein from Arabidopsis appears to be transcriptinally up-regulated by salicylic acid and NPR1-dependent mechanisms. J. Gen. Plant Pathol.
74: 345-354.
DOI: 10.1007/s10327-008-0117-1

Marten, H., Hyun, T., Gomi, K., Seo, S., Hedrich, R., and Roelfsema, M.R.G. (2008) Silencing of NtMPK4 impairs CO2-induced stomatal closure, activation
of anion channels and cytosolic Ca2+-signals in Nicotiana tabacum guard cells. Plant J. 55: 698-708.

Mitsuhara, I., Iwai, T., Seo, S., Yanagawa, Y., Kawahigasi, H., Hirose, S., Ohkawa, Y., and Ohashi, Y (2008) Characteristic expression of twelve rice PR1 family genes in response to pathogen infection, wounding, and defense-related signal compounds. Mol. Genet. Genomics 279: 415-427.

Yara, A., Yaeno, T., Montillet, J.-L., Hasegawa, M., Seo, S., Kusumi, K., and Iba, K. (2008) Enhancement of disease resistance to Magnaporthe grisea in rice by accumulation of hydroxy linoleic acid. Biochem. Biophys. Res. Commun. 370: 344-347.


Hibi, T., Kosugi, S., Iwai, T., Kawata, Seo, S., Mitsuhara, I., and Ohashi, Y. (2007) Involvement of EIN3 homologues in basic PR gene expression and flower development in tobacco plants. J. Exp. Bot. 58: 3671-3678.

Iwai, T. Seo, S., Mitsuhara, I. and Ohashi, Y. (2007) Probenazole-induced accumulation of salicylic acid confers resistance to Magnaporthe grisea in adult rice plants. Plant Cell Physiol. 48: 915-924.

Katou, S., Kuroda, K., Seo, S., Yanagawa, Y., Tsuge, T., Yamazaki, M., Miyao, A., Hirochika, H., Ohashi, Y. (2007) A calmodulin-binding mitogen-activated protein kinase phosphatase is induced by wounding and regulates the activities of stress-related mitogen-activated protein kinases in rice. Plant Cell Physiol. 48: 332-344.

Kawahigashi, H., Hirose, S., Iwai, T., Ohashi, Y., Sakamoto, W., Maekawa, M., and Ohkawa, Y. (2007) Chemically induced expression of rice OSB2 under the control of the OsPR1.1 promoter confers increased anthocyanin accumulation in transgenic rice. J. Agric. Food Chem. 55: 1241-1247.
DOI: 10.1021/jf062339w

Obara, N., Mistuhara, I., Seo, S., Ohashi, Y. and Hasegawa, M., and Matsuura, Y. (2007) Mechanism of PR gene expression by treatment of tobacco leaves with yeast extract (AGREVO EX). Jpn J. Phytopathol. 73: 94-101.
DOI: 10.3186/jjphytopath.73.94

Sasaki, K., Yuichi, O., Hiraga, S., Gotoh, Y., Seo, S., Mitsuhara, I., Ito, H., Matsui, H., and Ohashi, Y. (2007) Characterization of two rice peroxidase promoters that respond to blast fungus-infection. Mol. Genet. Genomics 278: 709-722.
DOI: 10.1007/s00438-007-0286-1

Sasaki, K., Mitsuhara, I., Seo, S., Ito, H., Mastui, H., and Ohashi, Y. (2007)
Two novel AP2/ERF domain proteins interact with cis-element VWRE for wound-induced expression of the Tobacco tpoxN1 gene. Plant J. 50: 1079-1092.

Seo, S.*, Katou, S.*, Seto, H., Gomi, K., and Ohashi, Y. (2007) The mitogen-activated protein kinases WIPK and SIPK regulate the levels of jasmonic and salicylic acids in wounded tobacco plants. Plant J. 49: 899-909. (*co-first authors)
DOI: 10.1111/j.1365-313X.2006.03003.x

Takabatake, R.*, Karita, E.*, Seo, S., Mitsuhara, I., Kuchitsu, K., and Ohashi, Y. (2007) Pathogen-induced calmodulin isoforms in basal resistance against bacterial and fungal pathogens in tobacco. Plant Cell Physiol. 48: 414-423. (*co-first authors)

akabatake, R., Ando, Y., Seo, S., Katou, S., Tsuda, S., Ohashi, Y. and Mitsuhara, I. (2007) MAP kinases function at the downstream of HSP90 and upstream of mitochondria in TMV-resistance gene N -mediated hypersensitive cell death. Plant Cell Physiol. 48: 498-510.

Takahashi, F., Yoshida, R., Ichimura, K., Mizoguchi, T., Seo, S., Yonezawa, M., Maruyama, K., Yamaguchi-Shinozaki, K., and Shinozaki, K. (2007) Dissecting the jasmonate signaling pathway by a novel MAP kinase cascade, MKK3-MPK6, in Arabidopsis. Plant Cell 19: 805-818

Yara, A., Yaeno, T., Hasegawa, M., Seto, H., Montillet, J.-L., Kusumi, K., Seo, S., and Iba, K. (2007) Disease resistance against Magnaporthe grisea is enhanced in transgenic rice with suppression of ω-3 fatty acid desaturases. Plant Cell Physiol. 48: 1263-1274.


Iwai, T., Miyasaka, A, Seo, S., and Ohashi, Y. (2006) Contribution of ethylene biosynthesis for resistance to blast fungus infection in young rice plants. Plant Physiol. 142: 1202-1215.
DOI: 10.1104/pp.106.085258

Kuroda, K., Sasaki, K., Iwai, T., Yazaki, Y., Hiraga, S., Seo, S., Mitsuhara, I., Minami, E., and Ohashi, Y. (2006) Rapid defense gene expression in both resistant and susceptible rice cultivars by elicitor(s) originating from conidia of blast fungus -basal resistance response before fungal penetration into host cells. Physiol. Mol. Plant Pathol. 69: 13-25.
DOI: 10.1016/j.pmpp.2006.12.003

Mitsuhara, I., Yatou, O., Iwai, T., Naito, Y., Nawa, Y., and Ohashi, Y. (2006) Genetic studies of transgenic rice plants overproducing an antibacterial peptide show that a high level of transgene expression did not cause inferior effects on host plants. Plant Biotechnol. 23: 63-69.

Ogawa, D., Nakajima, N., Seo, S., Mitsuhara, I., Kamada, H., and Ohashi, Y. (2006) The phenylalanine pathway is the main route of salicylic acid biosynthesis in Tobacco mosaic virus-infected tobacco leaves. Plant Biotechnol. 23: 395-398.

Sasaki, K., Ito, H., Mitsuhara, I., Hiraga, S., Seo, S., Matsui, H., and Ohashi, Y. (2006) A novel wound-responsive cis-elements, VWRE, of the vascular system-specific expression of a tobacco peroxidase gene, tpoxN1. Plant Mol. Biol. 62: 753-768.
DOI: 10.1007/s11103-006-9055-5

Takabatake, R., Seo, S., Ito, N., Gotoh, Y., Mitsuhara, I., and Ohashi, Y. (2006) Involvement of wound-induced receptor-like protein kinase in wound signal transduction in tobacco plants. Plant J. 47: 249-257.

Takabatake, R., Seo, S., Mitsuhara, I., Tsuda, S., and Ohashi, Y. (2006) Accumulation of the two transcripts for the N gene, conferring resistance to Tobacco mosaic virus, is likely important for N gene-dependent hypersensitive cell death. Plant Cell Physiol. 47: 254-261.


Gomi, K., Ogawa, D., Katou, S., Kamada, H., Nakajima, N., Saji, H., Soyano, T., Sasabe, M., Machida, Y., Mitsuhara, I., Ohashi, Y., and Seo, S. (2005) A mitogen-activated protein kinase NtMPK4 activated by SIPKK Is required for jasmonic acid signaling and involved in ozone tolerance via stomatal movement in tobacco. Plant Cell Physiol. 46: 1902-1914.

Katou, S., Yamakawa, H., Karita, E., Seo, S., Mitsuhara, I., Kuchitsu, K., and Ohashi, Y. (2005) Catalytic activation of plant MAPK phosphatase NtMKP1 by its physiological substrate SIPK, but not by calmodulins. J. Biol. Chem. 280: 39569-39581.

Shirasawa-Seo, N., Sano, Y, Nakamura, S., Murakami, T., Seo, S., Ohashi, Y., Hashimoto, Y., and Matsumoto, T. (2005) Characteristics of the promoters derived from the single-stranted DNA components of Milk vetch dwarf virus in transgenic tobacco. J. Gene. Virol. 86: 1851-1860.
DOI: 10.1099/vir.0.80790-0

Shirasawa-Seo, N., Sano, Y, Nakamura, S., Murakami, T, Gotoh, Y., Naito, Y., Hsia, C.-N., Seo, S., Mitsuhara, I., and Kosugi, S., and Ohashi, Y. (2005) The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants. Plant Cell Rep. 24: 155-163.


Ohashi, Y., Mitsuhara, I., Murakami, T., and Seo, S. (2004) Rapid down and upward translocation of salicylic acid and in tobacco plants. Plant Biotechnol. 21: 95-101.

Sasaki, K., Iwai, T., Hiraga, S., Kuroda, K., Seo, S., Mitsuhara, I., Miyasaka, A., Iwano, M., Ito, H., Matsui, H., and Ohashi, Y. (2004) Ten rice peroxidases redundantly respond to multiple stresses including infection with rice blast fungus. Plant Cell Physiol. 45: 1442-1452.

Yamakawa, H., Katou, S., Seo, S., Mitsuhara, I., Kamada, H., and Ohashi, Y. (2004) Plant mitogen-activated protein kinase phosphatase interacts with calmodulins. J. Biol. Chem. 279: 928-936.


Kosugi, S., and Ohashi, Y. (2003) Constitutive E2F expression in tobacco plants exhibits altered cell cycle control and morphological chnage in a cell type-specific manner. Plant Physiol. 132: 2012-2022.

Takahashi, Y., Berberich, T., Miyazaki, A., Seo, S., Ohashi, Y., and Kusano, T. (2003) Spermine signalling in tobacco: activation of mitogen-activated protein kinases by spermine is mediated through mitochondrial dysfunction. Plant J. 36: 820-829.

Seo, S., Seto, H., Koshino, H., Yoshida, S., and Ohashi, Y. (2003) A diterpene as an endogenous signal for the activation of defense responses to tobacco mosaic virus infection and wounding in tobacco. Plant Cell 15: 863-873.


Ito, N., Takabatake, R., Seo, S., Hiraga, S., Mitsuhara, I., and Ohashi, Y. (2002) Induced expression of a temperature-sensitive leucine-rich repeat receptor-like protein kinase gene by hypersensitive cell death and wounding in tobacco plant carrying the N resistance gene. Plant Cell Physiol. 43: 266-274.

Iwai, T., Kaku, H., Honkura, R., Nakamura, S., Ochiai, H., Sasaki, T., and Ohashi, Y. (2002) Enhanced resistance to seed-transmitted bacterial diseases in transgenic rice plants overproducing an oat cell-wall-bound thionin. Mol. Plant-Microbe Interact. 15: 515-521.

Kosugi, S. and Ohashi, Y. (2002) E2F sites that can interact with E2F proteins cloned from rice are required for meristematic tissue-specific expression of rice and tobacco proliferating cell nuclear antigen promoters. Plant J. 29: 45-59.

Kosugi, S. and Ohashi, Y. (2002) DNA binding and dimerization specificity and potential targets for the TCP protein family. Plant J. 30: 337-348.

Kosugi, S. and Ohashi, Y. (2002) E2Ls, E2F-like repressors of Arabidopsis that bind to E2F sites in a monomeric form. J. Biol. Chem. 277: 16553-16558.

Kosugi, S. and Ohashi, Y. (2002) Interaction of the Arabidopsis E2F and DP proteins confers their concomitant nuclear translocation and transactivation. Plant Physiol. 128: 833-843. DOI: 10.1104/pp/010642.    

Mitsuhara, I., Shirasawa-Seo, N., Iwai, T., Nakamura, S., Honkura, R., and Ohashi, Y. (2002) Release from post-transcriptional gene silencing by cell proliferation in transgenic tobacco plants: Possible mechanism for noninheritance of the silencing. Genetics 160: 343-352.

Qiao, J., Mitsuhara, I., Yazaki, Y., Sakano, K., Gotoh, Y., Miura, M., and Ohashi, Y. (2002)  Enhanced resistance to salt, cold and wound stresses by overproduction of animal cell death suppressors Bcl-xL and Ced-9 in tobacco cells - Their possible contribution through improved function of organelle. Plant Cell Physiol. 43: 992-1005.

Sasaki, K., Hiraga, S., Ito, H., Seo, S., Matsui, H., and Ohashi, Y. (2002) A wound-inducible tobacco peroxidase gene expresses preferentially in the vascular system. Plant Cell Physiol. 43: 108-117.

Shirasawa-Seo, N., Mitsuhara, I., Nakamura, S., Murakami, T., Iwai, T., Nishizawa, Y., Hibi, T., and Ohashi, Y. (2002) Constitutive promoters available for transgene expression instead of CaMV 35S RNA promoter: Arabidopsis promoters of tryptophan synthase protein β subunit and phytochrome B. Plant Biotechnol. 19: 19-26.

Shirasawa-Seo, N., Nakamura, S., Ukai, N., Honkura, R., Iwai, T., and Ohashi, Y. (2002) Ectopic expression of an oat thionin gene in carnation plants confers enhanced resistance to bacterial wilt disease. Plant Biotechnol. 19: 311-317.


Seo, S., Seto, H., Yamakawa, H., and Ohashi, Y. (2001) Transient accumulation of jasmonic acid during the synchronized hypersensitive cell death in tobacco mosaic virus-infected tobacco leaves. Mol. Plant-Microbe Interact. 14: 261-264.

Yamakawa, H., Mitsuhara I., Ito, N., Seo, S., Kamada, H., and Ohashi, Y. (2001) Transcriptionally and post-transcriptionally regulated response of 13 calmodulin genes to tobacco mosaic virus-induced cell death and wounding in tobacco plant. Eur. J. Biochem. 268: 3916-3929.

Watanabe, T., Seo, S., and Sakai, S. (2001) Wound-induced expression of a gene for 1-aminocyclopropane-1-carboxylate synthase and ethylene production are regulated by both reactive oxygen species and jasmonic acid in Cucurbita maxima. Plant Physiol. Biochem. 39: 121-127.


Fujibe, T., Watanabe, K., Nakajima, N., Ohashi, Y., Mitsuhara, I., Yamamoto, K.T., and Takeuchi, Y. (2000) Accumulation of pathogenesis-related proteins in tobacco leaves irradiated with UV-B. J. Plant Res. 113: 387-394.

Fukuoka, H., Ogawa, T., Mitsuhara, I., Iwai, T., Isuzugawa, K., Nishizawa, Y., Gotoh, Y., Nishizawa, Y., Tagiri, A., Ugaki, M., Ohshima, M., Yano, H., Murai, N., Niwa, Y., Hibi, T., and Ohashi, Y. (2000) Agrobacterium-mediated transformation of monocot and dicot plants using the NCR promoter derived from soybean chlorotic mottle virus. Plant Cell Rep. 19: 815-820.

Hiraga, S., Ito, H., Yamakawa, H., Ohtsubo, N., Seo, S., Mitsuhara, I., Matsui, H., Honma, M., and Ohashi, Y. (2000) An HR-induced tobacco peroxidase gene is responsive to spermine, but not to salicylate, methyl jasmonate, and ethephon. Mol. Plant-Microbe Interact. 13: 210-216.

Hiraga, S., Yamamoto, K., Ito, H., Sasaki, K., Matsui, H., Honma, M., Nagamura, Y., Sasaki, T., and Ohashi, Y. (2000) Diverse expression profiles of 21 rice peroxidase genes. FEBS Lett. 471: 245-250.

Hiraga, S., Ito, H., Sasaki, K., Yamakawa, H., Mitsuhara, I., Toshima, H., Matsui, H., Honma, M., and Ohashi, Y. (2000) Wound-induced expression of a tobacco peroxidase is not enhanced by ethephon and suppressed by methyl jasmonate and coronatine. Plant Cell Physiol. 41: 165-170.

Ito, H., Hiraga, S., Tsugawa, H., Matsui, H., Honma, M., Otsuki, Y., Murakami, T., and Ohashi, Y. (2000) Xylem-specific expression of wound-inducible rice peroxidase genes in transgenic plants. Plant Sci. 155: 85-100.

Kodama, H., Nishiuchi, T., Seo, S., Ohashi, Y., and Iba, K. (2000) Possible involvement of protein phosphorylation in the wound responsive expression of Arabidopsis plastid ω-3 fatty acid desaturase gene. Plant Sci. 155: 153-160.

Kosugi, S. and Ohashi, Y. (2000) Cloning and DNA-binding properties of a tobacco Ethylene-Insensitive3 (EIN3) homolog. Nucl. Acids Res. 28: 960-967.

Mitsuhara, I., Matsufuru, H., Ohshima, M., Kaku, H., Nakajima, Y., Murai, N., Natori, S., and Ohashi, Y. (2000) Induced expression of sarcotoxin IA enhanced host resistance against both bacterial and fungal pathogens in transgenic tobacco. Mol. Plant-Microbe Interact. 13: 860-868.

Seo, S., Okamoto, M., Iwai, T., Iwano, M., Fukui, K., Isogai, A., Nakajima, N., and Ohashi, Y. (2000) Reduced levels of chloroplast FtsH protein in tobacco mosaic virus-infected tobacco leaves accelerate the hypersensitive reaction. Plant Cell 12: 917-932.

Ueda, T., Seo, S., Ohashi, Y., and Hashimoto, J. (2000) Circadian and senescence-enhanced expression of a tobacco cystein protease gene. Plant Mol. Biol. 44: 649-657.


Hiraga, S., Ito, H., Matsui, H., Honma, M., and Ohashi, Y. (1999) cDNA Sequences for two novel tobacco peroxidase isoenzymes. Plant Physiol. 120: 1205-1207. PGR 99-109.

Ito, N., Seo, S., Ohtsubo, N., Nakagawa, H., and Ohashi, Y. (1999) Involvement of proteasome-ubiquitin system in wound-signaling in tobacco plants. Plant Cell Physiol. 40: 355-360.

Mitsuhara, I., Malik, K.A., Miura, M., and Ohashi, Y. (1999) Animal cell-death suppressors Bcl-xL and Ced-9 inhibit cell death in tobacco plants. Curr. Biol. 9: 775-778.

Ohshima, M., Mitsuhara, I., Okamoto, M., Sawano, S., Nishiyama, K., Kaku, H., Natori, S., and Ohashi, Y. (1999) Enhanced resistance to bacterial diseases in transgenic tobacco leaf overexpressing sarcotoxin IA, a bactericidal peptide of insect. J. Biochem. 125: 431-435.

Ohtsubo, N., Mitsuhara, I., Koga, M., Seo, S., and Ohashi, Y. (1999) Ethylene promotes the necrotic lesion formation and basic PR gene expression in TMV-infected tobacco. Plant Cell Physiol. 40: 808-817.

Seo, S ., Sano, H., and Ohashi, Y. (1999) Jasmonate-based wound signal transduction requires activation of WIPK, a tobacco mitogen-activated protein kinase. Plant Cell 11: 289-298. DOI: 10.1105/tpc/11.2.289

Yanagawa, Y., Ohashi, Y., Murakami, Y., Saeki, H., Yokosawa, K., Tanaka, Hashimoto, J., Sato, T., and Nakagawa, H. (1999) Purification and characterization of the 26S proteasome from cultured rice(Oryza sativa) cells. Plant Sci. 149: 33-41.


Niki, T., Mitsuhara, I., Seo, S., Ohtsubo, N., and Ohashi, Y. (1998) Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) protein genes in wounded mature tobacco leaves. Plant Cell Physiol. 39: 500-507.

Yamakawa, H., Kamada, H., Satoh, M., and Ohashi, Y. (1998) Spermine is a salicylate-independent endogenous inducer for both tobacco acidic pathogenesis-related proteins and resistance against tobacco mosaic virus infection. Plant Physiol. 118: 1213-1222.


Kosugi, S. and Ohashi, Y. (1997) PCF1 and PCF2 specifically bind to cis elements in the rice proliferating cell nuclear antigen gene. Plant Cell 9: 1607-1609.

Nakamura, S., Iwai, T., Honkura, R., Ugaki, M., Ohshima, M., and Ohashi, Y. (1997) Four chitinase cDNAs from Chenopodium amaranticolor. Plant Biotechnol. 14: 85-86.


Mitsuhara, I., Ugaki, M., Hirochika, H., Ohshima, M., Murakami, T., Gotoh, Y., Katayose, Y., Nakamura, S., Honkura, R., Nishimiya, S., Ueno, K., Mochizuki, A., Tanimoto, H., Tsugawa,  H., Otsuki, Y., and Ohashi, Y. (1996) Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol. 37: 49-59.

Ohashi, Y. (1996) Molecular breeding for resistant transgenic plants against pathogen attack. Gamma Field Symposia 35: 97-106.

Sano, H., Seo, S., Koizumi, N., Niki, T., Iwamura, H., and Ohashi, Y. (1996) Regulation by cytokinins of endogenous levels of jasmonic and salicylic acids in mechanically wounded tobacco plants. Plant Cell Physiol. 37: 762-769.


Kosugi, S., Suzuka, I. and Ohashi, Y. (1995) Two of three promoter elements identified in a rice gene for proliferating cell nuclear antigen are essential for meristematic tissue-specific expression. Plant J. 7: 877-886.

Nakamura, S., Honkura, R., Ugaki, M., Ohshima, M., and Ohashi, Y. (1995) Resistance to viral infection in transgenic plants expressing ribozymes disigned against cucumber mosaic virus RNA. Ann. Phytopathol. Soc. Jpn. 61: 53-55.

Sano, H. and Ohashi, Y. (1995) Involvement of small GTP-binding proteins in defense signal-transduction pathways of higher plants. Proc. Natl. Acad. Sci. USA 92: 4138-4144.

Seo, S ., Okamoto, M., Seto, H., Ishizuka, K., Sano, H., and Ohashi, Y. (1995) Tobacco MAP kinase: A possible mediator in wound signal transduction pathways. Science 270: 1988-1992.

Seo, S ., Ishizuka, K., and Ohashi, Y. (1995) Induction of salicylic acid ß-glucosidase in tobacco leaves by exogenous salicylic acid. Plant Cell Physiol. 36: 447-453.


Itoh, H., Kimzuka, F., Ohbarashi, A., Matsui, H., Honma, M., Shinmyo, A., Ohashi, Y., Caplan, A.B., and Rodriguez, R.L. (1994) Molecular cloning and characterization of two complementary DNAs encoding putative peroxidases from rice (Oryza sativa L.) shoot. Plant Cell Rep. 13: 361-366.

Kishimoto, K., Itoh, H., Ohashi, Y., and Saito, A. (1994) RFLP mapping of a coding sequence for rice peroxidase: detection of a locus different from those determined by isozyme analysis. Jpn. J. Genet. 69: 101-104.

Nakamura, S., Honkura, R., Ugaki, M., Ohshima, M., and Ohashi, Y. (1994) Nucleotide sequence of the 3'-terminal region of bean yellow mosaic virus RNA and resistance to viral infection in transgenic Nicotiana benthamiana expressing its coat protein gene. Ann. Phytopathol. Soc. Jpn. 60: 295-304.

Sano, H., Seo, S., Orudgev, E., Youssefian, S., Ishizuka, K., and Ohashi, Y. (1994) Expression of the gene for a small GTP binding protein in transgenic tobacco elevates endogenous cytokinin levels, abnormally induces salicylic acid in response to wounding, and increases response to tobacco mosaic virus infection. Proc. Natl. Acad. Sci. USA 91: 10556-10560.

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Reviews and books

Note: Only reviews and books written in English are listed.

Ohashi, Y., Seo, S., Mitsuhara, I., Yamakawa, H., and Takabatake, R. (2004). Signal transduction in TMV-infected and wounded tobacco plants. Genomic and Genetic Analysis of Plant Parasitism and Defense. Tsuyumu, S., Leach, J.E., Shiraishi, T., and Wolpert, T., ed., APS press 249-257.
ISBN: 978-0-89054-323-8

MAPK Group (2002) Mitogen-activated protein kinase cascades in plants: a new nomenclature. Trends Plant Sci. 7: 301-308.

Hiraga, S., Sasaki, K., Ito, H., Ohashi, Y., and Matsui, H. (2001) A large family of class III plant peroxidases. Plant Cell Physiol. 42: 462-468.

Ohashi, Y., Seo, S., Mitsuhara, I. Yamakawa, H., and Ito, N. (2001) Signaling Pathways for TMV- and Wound-Induced Resistance in Tobacco Plants. Delivery and Perception of Pathogen signals in Plants. Keen, N.T., Mayama, S., Leach, J.E., and Tsuyumu, S., ed., APS press 122-129.
ISBN: 978-0-89054-259-0

Seo, S. and Ohashi, Y. (1999) Mitogen-activated protein kinases and wound stress. Results and Problems in Cell Differentiation. Hirt, H., ed.: MAP Kinases in Plant Signal Transduction. Springer-Verlag, Berlin, Vol. 27, 53-63.
ISBN: 9783540656258

Seo, S., Sano, H., and Ohashi, Y. (1997) Jasmonic acid in wound signal transdunction pathways. Physiol. Plant. 101: 740-745.

Seo, S., Sano, H., and Ohashi, Y. (1997) Transgenic manipulation of signaling pathways of plant resistance to pathogen attack. Biotechnol. Ann. Rev. 3: 197-225.

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2007 Young Researcher Award of Agriculture, Forestory, and Fisheries for Shigemi Seo

2006 Young Scientist Award of Science and Technology Minister for Shigemi Seo

2005 JSPCMB Excellent Paper Award for our paper (Ohashi et al. (2004) Plant Biotechnol. 21: 95-101.

2001 The JSPP Plant and Cell Physiology Award for the Paper of Excellence for two our papers (Niki et al. Plant Cell Physiol. (1998) 39: 500-507; Ohtsubo et al.  (1999) Plant Cell Physiol. 40: 808-817)

1997 Science and Technology Minister Award for Yuko  Ohashi

1996 Winner of the Phytopathological Society of Japan Fellowship for Yuko Ohashi

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