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小澤様
 生物研の山本です。以下の手順で冷蔵保護すると良いでしょう。20℃:3〜4日
間、15℃:3〜4日間、8〜10℃:3〜4日間、保護した後、0〜5℃に3ヶ月冷蔵す
る。(産卵後の高温期間が1ヶ月以上になると、中間温度の保護期間をもう少し長く
する必要があります)。なお、一般に休眠卵(越年)の形態で短期間で孵化させる場
合は、産卵後の高温期間を10日間程度にとどめることが重要ですので、今後の参考
にして下さい。
 催青方法は通常の方法で行います:8〜10℃:2日間、15℃:1〜2日間、経過させ
た後、25℃で催青して孵化させる。なお、即時浸酸用の塩酸液に5分程度浸漬すれ
ば、孵化率を高めることができます。
 
----- Original Message -----
From: ozawa
To: silkmail@ml.affrc.go.jp
Sent: Sunday, February 08, 2004 2:51 PM
Subject: [silkmail:181] Re: 教えてください


山本様

突然のメールで失礼します。

メールありがとうございました。

今回アドバイス頂いた三ヶ月冷蔵する方法を試してみたいと思います。

そこで、孵化までの詳しい方法をお教えいただければ幸いです。



小澤隆二



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