National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2004

18. Molecular typing of Staphylococcus aureus isolated from mastitis on the basis of coagulase gene polymorphisms

Japanese

This study was conducted to investigate the polymorphism of the coagulase gene of Staphylococcus aureus causing bovine mastitis. A total of 270 strains of Staphylococcus aureus were isolated from mastitic milk obtained from 175 different dairy farms. The isolates were characterized for restriction fragment length polymorphism of the coagulase gene. Coagulase genotyping revealed 14 patterns, and 161 of the 270 isolates (59.6%) belonged to the coagulase genotype B1. Further, these 161 isolates possessed at least two enterotoxin genes. Coagulase genotyping provides more detailed epidemiological information on S. aureus, and this PCR-based genotyping method may be an important tool for typing Staphylococcus aureus.
(Environmental Hygiene Section, Department of Epidemiology TEL +81-176-62-5115)

Reference:

Katsuda et al. (2005) Vet. Microbiol.105:301-305.

 

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