National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2006

03. Development of rapid and sensitive detection method for Theileria orientalis sergenti infection rates in Haemaphysalis longicornis by polymerase chain reaction

  Haemaphysalis longicornis has been recognized as the most important vector tick of Theileria orientalis sergenti (TOS) in Japan. A rapid and sensitive detection method for TOS infection rates in Haemaphysalis longicornis was developed using a polymerase chain reaction. This method could detect the TOS gene from a single nymphal or adult tick. The result of detecting TOS infection rates by this method correlated with the methyl green-pyronin staining method, which is a method for detecting TOS infection rates in ticks. Consequently, it is possible to use this method for diagnosing TOS infection rates of ticks and forecasting the outbreak of TOS infection in a grazing pasture.
(Research Team for Environmental/Enzootic Diseases, Tohoku Research Station, TEL +81-176-62-5115)

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