National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2006

13. A rapid test for assessment of scrapie prion inactivation

  Abnormal isoforms of prion proteins (PrPSc), which are infectious agents associated with prion diseases, retain infectivity after undergoing routine sterilization processes. A sensitive method for detecting the infectivity is a bioassay, and it has been used for assessing prion inactivation. However, the result is obtained after several hundred days. Here, protein misfolding cyclic amplification (PMCA), in which PrPSc can be amplified in vitro, was applied to assess prion inactivation by dry heating and autoclaving. Scrapie-infected hamster brains were inactivated under various conditions, and residual infectivity and PrPSc were detected by bioassay and PMCA, respectively. The PMCA results were in good agreement with those of the bioassay. In samples autoclaved at temperatures below 150ºC, while infected mice died in the bioassay, protease-resistant PrP (PrPres) signals were detected in the second or third round of PMCA. Three rounds of PMCA require only 6 days, which means that the PMCA method is much faster than the bioassay.
(Prion Research Team, Prion Disease Research Center, TEL +81-29-838-8333)

Reference:

Murayama et al. (2006) Biochem. Biophys. Res. Commun. 348 : 758-762.

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