We have established multiplex polymerase chain reaction assays to identify 7 serovars of Salmonella—i.e. Typhimurium, Choleraesuis, Infantis, Hadar, Enteritidis, Dublin, and Gallinarum. Three serovar-specific genomic regions (SSGRs) were selected in each serovar using a comparative genomics approach. The Salmonella-specific invA was used to confirm the genetic background of the organisms. The isolates tested were identified as a target serovar when the 3 selected SSGRs and invA were all positive for amplification. These assays had sufficient specificity to identify the 7 Salmonella serovars, and therefore, have the potential for use for rapid screening.
(Bacterial and Parasitic Disease Research Division)
References:
Akiba M. et al (2011) J. Microbiol. Methods. 85:9-15