National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2011

22. Rapid identification of Salmonella enterica serovars Typhimurium, Choleraesuis, Infantis, Hadar, Enteritidis, Dublin, and Gallinarum using multiplex PCR

  We have established multiplex polymerase chain reaction assays to identify 7 serovars of Salmonella—i.e. Typhimurium, Choleraesuis, Infantis, Hadar, Enteritidis, Dublin, and Gallinarum. Three serovar-specific genomic regions (SSGRs) were selected in each serovar using a comparative genomics approach. The Salmonella-specific invA was used to confirm the genetic background of the organisms. The isolates tested were identified as a target serovar when the 3 selected SSGRs and invA were all positive for amplification. These assays had sufficient specificity to identify the 7 Salmonella serovars, and therefore, have the potential for use for rapid screening.
(Bacterial and Parasitic Disease Research Division)

References:

Akiba M. et al (2011) J. Microbiol. Methods. 85:9-15

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