National Institute of Animal Health (NIAH)

Topics in Animal Health Research 2012

19. Immunostimulatory effects of recombinant Erysipelothrix rhusiopathiae expressing porcine interleukin-18 in mice and pigs

Japanese

Interleukin-18 (IL-18), which was originally called gamma interferon (IFN-γ)-inducing factor, has been shown to play an important role in innate and acquired immune responses. In this study, attenuated Erysipelothrix rhusiopathiae strains were engineered to produce porcine IL-18 (poIL-18) and evaluated for their potential immunostimulatory effect in animals. Recombinant poIL-18 was successfully expressed in the recombinant E. rhusiopathiae strains YS-1/IL-18 and KO/IL-18. The culture supernatant of YS-1/IL-18 was confirmed to induce IFN-γ production in murine splenocytes in vitro, and this production was inhibited by incubation with anti-poIL 18 monoclonal antibodies. Furthermore, greater IFN-γ production was induced upon stimulation of splenocytes with concanavalin A when using splenocytes from mice that were intraperitoneally inoculated with YS-1/IL-18 than when using splenocytes from control mice inoculated with the parent strain YS-1. Peritoneal macrophages from mice pre-inoculated with YS-1/IL-18 exhibited enhanced phagocytosis of Salmonella enterica subsp. enterica serovar Typhimurium compared with peritoneal macrophages from control mice pre-inoculated with YS-1. We also confirmed the immunostimulatory effect on humoral immune responses against antigens of E. rhusiopathiae and Mycoplasma hyopneumoniae in gnotobiotic pigs that were orally pre-inoculated with KO/IL-18. Thus, these results provide evidence that E. rhusiopathiae is a promising vector for the expression of host cytokines and suggest the potential utility of E. rhusiopathiae vector-encoded cytokines in the activation of host innate and acquired immune responses.
(Bacterial and Parasitic Disease Research Division)

References:

Ogawa Y. et al. (2012) Clin. Vaccine Immunol. 19(9):1393-1398

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