生物機能利用研究部門

有用物質生産作物開発ユニット

役割

バイオテクノロジーを活用し、様々な生物由来の健康機能性成分、医薬品成分等の有用物質を産生する新機能作物を開発し、我が国の農業の国際競争力強化や、消費者の生活の質 (QOL) の向上に貢献します。

主な研究テーマ

スギ花粉米の社会実装
医薬品のような有用物質を生産するイネの開発
アレルギー疾患や生活習慣病の予防・治療効果を付与した健康機能性米の開発
イネ種子における組換えタンパク質の高度集積法に関する研究
葉緑体形質転換を介した有用物質生産タバコ・レタスの開発

生物由来の有用物質は、その設計図となる遺伝子を生産に適した生物に導入することで収量や品質の向上と効果的な利用が可能になります。私たちは、農作物を有用物質生産の場として選択し、有用物質と農作物の新しい利用法の開拓を試みています。例えば、私たちが開発した技術により、お米の細胞内にある特別な顆粒中に有用タンパク質をうまく蓄積させると、食べたお米が天然のカプセル剤となり、目的の消化器官まで有用タンパク質を運んでくれます(図)。このような技術と新しい作物の開発に取り組んでいます。

腸管粘膜組織へのタンパク質の効率的なデリバリー機能

タンパク質顆粒(PB-I、PB-II )に組換えタンパク質(スギ花粉抗原など)を蓄積させると、胃では消化されずに腸管免疫系に到達し、免疫寛容 (アレルギー反応の低下) などの機能性を誘導しやすくなると考えられています。


メンバー

ユニット長
小沢憲二郎
上級研究員
高木英典
主任研究員
川勝泰二
若佐雄也
任期付研究員
林 晋平

メンバーの主要成果

「スギ花粉ポリペプチド含有米」及び「スギ花粉ペプチド含有米」の研究を目的とした外部機関への提供について

主要成果

原著論文

  • Nanasato Y, Namiki S, Oshima M, Moriuchi R, Konagaya K-I, Seike N, et al. Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA. Plant Cell Rep. 2016 Sep;35(9):1963-74.
  • Takaiwa F, Yang L, Maruyama N, Wakasa Y, Ozawa K. Deposition mode of transforming growth factor-β expressed in transgenic rice seed. Plant Cell Rep. 2016 Aug;
  • Kawakatsu T, Huang S-SC, Jupe F, Sasaki E, Schmitz RJ, Urich MA, et al. Epigenomic Diversity in a Global Collection of Arabidopsis thaliana Accessions. Cell. 2016 Jul;166(2):492-505.
  • Hayashi S, Wakasa Y, Ozawa K, Takaiwa F. Characterization of IRE1 ribonuclease-mediated mRNA decay in plants using transient expression analyses in rice protoplasts. New Phytol. 2016 Jun;210(4):1259-68.
  • Ogo Y, Mori T, Nakabayashi R, Saito K, Takaiwa F. Transgenic rice seed expressing flavonoid biosynthetic genes accumulate glycosylated and/or acylated flavonoids in protein bodies. J Exp Bot. 2016 Jan;67(1):95-106.
  • Wakasa Y, Takaiwa F. Analysis of Recombinant Proteins in Transgenic Rice Seeds: Identity, Localization, Tolerance to Digestion, and Plant Stress Response. Methods Mol Biol. 2016;1385:223-47.
  • Takagi H, Takaiwa F. Production of Rice Seed-Based Allergy Vaccines. Methods Mol Biol. 2016;1403:713-21.
  • Kawakatsu T, Stuart T, Valdes M, Breakfield N, Schmitz RJ, Nery JR, et al. Unique cell-type-specific patterns of DNA methylation in the root meristem. Nat Plants. 2016;2(5):16058.
  • Shimosaka E, Ozawa K. Overexpression of cold-inducible wheat galactinol synthase confers tolerance to chilling stress in transgenic rice. Breed Sci. 2015 Dec;65(5):363-71.
  • Ohta M, Takaiwa F. OsHrd3 is necessary for maintaining the quality of endoplasmic reticulum-derived protein bodies in rice endosperm. J Exp Bot. 2015 Aug;66(15):4585-93.
  • Hayashi S, Takaiwa F. Visualization of endoplasmic reticulum stressed cells for forward genetic studies in plants. J Plant Physiol. 2015 May;180:61-6.
  • Wakasa Y, Takagi H, Watanabe N, Kitamura N, Fujiwara Y, Ogo Y, et al. Concentrated protein body product derived from rice endosperm as an oral tolerogen for allergen-specific immunotherapy--a new mucosal vaccine formulation against Japanese cedar pollen allergy. PLoS ONE. 2015;10(3):e0120209.
  • Nanasato Y, Tabei Y. Cucumber (Cucumis sativus L.) and kabocha squash (Cucurbita moschata Duch). Methods Mol Biol. 2015;1223:299-310.
  • Ogo Y, Wakasa Y, Hirano K, Urisu A, Matsuda T, Takaiwa F. Generation of transgenic rice with reduced content of major and novel high molecular weight allergens. Rice (N Y). 2014 Dec;7(1):19.
  • Yang L, Kawakatsu T, Wakasa Y, Yoine M, Takaiwa F. RNA silencing is induced by the expression of foreign recombinant products in transgenic rice. Plant Sci. 2014 Aug;225:138-46.
  • Takaiwa F, Yang L. Development of a rice-based peptide vaccine for Japanese cedar and cypress pollen allergies. Transgenic Res. 2014 Aug;23(4):573-84.
  • Takahashi H, Wang S, Hayashi S, Wakasa Y, Takaiwa F. Cis-element of the rice PDIL2-3 promoter is responsible for inducing the endoplasmic reticulum stress response. J Biosci Bioeng. 2014 May;117(5):620-3.
  • Wakasa Y, Oono Y, Yazawa T, Hayashi S, Ozawa K, Handa H, et al. RNA sequencing-mediated transcriptome analysis of rice plants in endoplasmic reticulum stress conditions. BMC Plant Biol. 2014;14:101.
  • Iizuka M, Wakasa Y, Tsuboi H, Asashima H, Hirota T, Kondo Y, et al. Prophylactic effect of the oral administration of transgenic rice seeds containing altered peptide ligands of type II collagen on rheumatoid arthritis. Biosci Biotechnol Biochem. 2014;78(10):1662-8.
  • Ohta M, Wakasa Y, Takahashi H, Hayashi S, Kudo K, Takaiwa F. Analysis of rice ER-resident J-proteins reveals diversity and functional differentiation of the ER-resident Hsp70 system in plants. J Exp Bot. 2013 Dec;64(18):5429-41.
  • Kawakatsu T, Kawahara Y, Itoh T, Takaiwa F. A whole-genome analysis of a transgenic rice seed-based edible vaccine against cedar pollen allergy. DNA Res. 2013 Dec;20(6):623-31.
  • Wakasa Y, Hayashi S, Takaiwa F. Effect of overexpression of kinase- or RNase-deficient OsIRE1 on the endoplasmic reticulum stress response in transgenic rice plants. Plant Signal Behav. 2013 Sep;8(9).
  • Ogo Y, Ozawa K, Ishimaru T, Murayama T, Takaiwa F. Transgenic rice seed synthesizing diverse flavonoids at high levels: a new platform for flavonoid production with associated health benefits. Plant Biotechnol J. 2013 Aug;11(6):734-46.
  • Nanasato Y, Konagaya K-I, Okuzaki A, Tsuda M, Tabei Y. Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks. Plant Biotechnol Rep. 2013 Jul;7(3):267-76.
  • Wang S, Takahashi H, Kajiura H, Kawakatsu T, Fujiyama K, Takaiwa F. Transgenic rice seeds accumulating recombinant hypoallergenic birch pollen allergen Bet v 1 generate giant protein bodies. Plant Cell Physiol. 2013 Jun;54(6):917-33.
  • Takaiwa F. Increasing the production yield of recombinant protein in transgenic seeds by expanding the deposition space within the intracellular compartment. Bioengineered. 2013 Jun;4(3):136-9.
  • Hayashi S, Takahashi H, Wakasa Y, Kawakatsu T, Takaiwa F. Identification of a cis-element that mediates multiple pathways of the endoplasmic reticulum stress response in rice. Plant J. 2013 Apr;74(2):248-57.
  • Takaiwa F. Update on the use of transgenic rice seeds in oral immunotherapy. Immunotherapy. 2013 Mar;5(3):301-12.
  • Kudo K, Ohta M, Yang L, Wakasa Y, Takahashi S, Takaiwa F. ER stress response induced by the production of human IL-7 in rice endosperm cells. Plant Mol Biol. 2013 Mar;81(4-5):461-75.
  • Wakasa Y, Yasuda H, Takaiwa F. Secretory type of recombinant thioredoxin h induces ER stress in endosperm cells of transgenic rice. J Plant Physiol. 2013 Jan;170(2):202-10.
  • Wakasa Y, Takagi H, Hirose S, Yang L, Saeki M, Nishimura T, et al. Oral immunotherapy with transgenic rice seed containing destructed Japanese cedar pollen allergens, Cry j 1 and Cry j 2, against Japanese cedar pollinosis. Plant Biotechnol J. 2013 Jan;11(1):66-76.
  • Yang L, Hirose S, Takahashi H, Kawakatsu T, Takaiwa F. Recombinant protein yield in rice seed is enhanced by specific suppression of endogenous seed proteins at the same deposit site. Plant Biotechnol J. 2012 Dec;10(9):1035-45.
  • Tsuda M, Okuzaki A, Kaneko Y, Tabei Y. Persistent C genome chromosome regions identified by SSR analysis in backcross progenies between Brassica juncea and B. napus. Breed Sci. 2012 Dec;62(4):328-33.
  • Wakasa Y, Ozawa K, Takaiwa F. Agrobacterium-mediated co-transformation of rice using two selectable marker genes derived from rice genome components. Plant Cell Rep. 2012 Nov;31(11):2075-84.
  • Wakasa Y, Hayashi S, Takaiwa F. Expression of OsBiP4 and OsBiP5 is highly correlated with the endoplasmic reticulum stress response in rice. Planta. 2012 Nov;236(5):1519-27.
  • Kawakatsu T, Takaiwa F. Reduction of 13 kD prolamins increases recombinant protein yield and recovery rate in rice endosperm. Plant Signal Behav. 2012 Nov;7(11):1402-3.
  • Kawakatsu T, Wakasa Y, Yasuda H, Takaiwa F. RNA silencing induced by an artificial sequence that prevents proper transcription termination in rice. Plant Physiol. 2012 Oct;160(2):601-12.
  • Tsuda M, Okuzaki A, Kaneko Y, Tabei Y. Relationship between hybridization frequency of Brassica juncea × B. napus and distance from pollen source (B. napus) to recipient (B. juncea) under field conditions in Japan. Breed Sci. 2012 Sep;62(3):274-81.
  • Yang L, Hirose S, Suzuki K, Hiroi T, Takaiwa F. Expression of hypoallergenic Der f 2 derivatives with altered intramolecular disulphide bonds induces the formation of novel ER-derived protein bodies in transgenic rice seeds. J Exp Bot. 2012 May;63(8):2947-59.
  • Ozawa K, Wakasa Y, Ogo Y, Matsuo K, Kawahigashi H, Takaiwa F. Development of an efficient agrobacterium-mediated gene targeting system for rice and analysis of rice knockouts lacking granule-bound starch synthase (Waxy) and β1,2-xylosyltransferase. Plant Cell Physiol. 2012 Apr;53(4):755-61.
  • Hayashi S, Wakasa Y, Takahashi H, Kawakatsu T, Takaiwa F. Signal transduction by IRE1-mediated splicing of bZIP50 and other stress sensors in the endoplasmic reticulum stress response of rice. Plant J. 2012 Mar;69(6):946-56.
  • Suzuki K, Yang L, Takaiwa F. Transgenic rice accumulating modified cedar pollen allergen Cry j 2 derivatives. J Biosci Bioeng. 2012 Feb;113(2):249-51.
  • Takahashi H, Kawakatsu T, Wakasa Y, Hayashi S, Takaiwa F. A rice transmembrane bZIP transcription factor, OsbZIP39, regulates the endoplasmic reticulum stress response. Plant Cell Physiol. 2012 Jan;53(1):144-53.
  • Wakasa Y, Takaiwa F. Use of a callus-specific selection system to develop transgenic rice seed accumulating a high level of recombinant protein. Methods Mol Biol. 2012;847:467-79.
  • Wakasa Y, Hayashi S, Ozawa K, Takaiwa F. Multiple roles of the ER stress sensor IRE1 demonstrated by gene targeting in rice. Sci Rep. 2012;2:944.
  • Pitakrattananukool S, Kawakatsu T, Anuntalabhochai S, Takaiwa F. Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice. Biosci Biotechnol Biochem. 2012;76(7):1296-302.
  • Ozawa K. A high-efficiency Agrobacterium-mediated transformation system of rice (Oryza sativa L.). Methods Mol Biol. 2012;847:51-7.
  • Hayashi S, Wakasa Y, Takaiwa F. Functional integration between defence and IRE1-mediated ER stress response in rice. Sci Rep. 2012;2:670.
  • Wakasa Y, Hirano K, Urisu A, Matsuda T, Takaiwa F. Generation of transgenic rice lines with reduced contents of multiple potential allergens using a null mutant in combination with an RNA silencing method. Plant Cell Physiol. 2011 Dec;52(12):2190-9.
  • Tsuda M, Konagaya K-I, Okuzaki A, Kaneko Y, Tabei Y. Occurrence of metaxenia and false hybrids in Brassica juncea L. cv. Kikarashina × B. napus. Breed Sci. 2011 Dec;61(4):358-65.
  • Suzuki K, Kaminuma O, Yang L, Takai T, Mori A, Umezu-Goto M, et al. Prevention of allergic asthma by vaccination with transgenic rice seed expressing mite allergen: induction of allergen-specific oral tolerance without bystander suppression. Plant Biotechnol J. 2011 Dec;9(9):982-90.
  • Wakasa Y, Zhao H, Hirose S, Yamauchi D, Yamada Y, Yang L, et al. Antihypertensive activity of transgenic rice seed containing an 18-repeat novokinin peptide localized in the nucleolus of endosperm cells. Plant Biotechnol J. 2011 Sep;9(7):729-35.
  • Hiroi T, Kaminuma O, Takaiwa F. Vaccination with transgenic rice seed expressing mite allergen: a new option for asthma sufferers? Expert Rev Vaccines. 2011 Sep;10(9):1249-51.
  • Nanasato Y, Konagaya K, Okuzaki A, Tsuda M, Tabei Y. Agrobacterium-mediated transformation of kabocha squash (Cucurbita moschata Duch) induced by wounding with aluminum borate whiskers. Plant Cell Rep. 2011 Aug;30(8):1455-64.
  • Wakasa Y, Tamakoshi C, Ohno T, Hirose S, Goto T, Nagaoka S, et al. The hypocholesterolemic activity of transgenic rice seed accumulating lactostatin, a bioactive peptide derived from bovine milk β-lactoglobulin. J Agric Food Chem. 2011 Apr;59(8):3845-50.
  • Okuzaki A, Konagaya K, Nanasato Y, Tsuda M, Tabei Y. Estrogen-inducible GFP expression patterns in rice (Oryza sativa L.). Plant Cell Rep. 2011 Apr;30(4):529-38.
  • Wakasa Y, Yasuda H, Oono Y, Kawakatsu T, Hirose S, Takahashi H, et al. Expression of ER quality control-related genes in response to changes in BiP1 levels in developing rice endosperm. Plant J. 2011 Mar;65(5):675-89.
  • Sato Y, Masuta Y, Saito K, Murayama S, Ozawa K. Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa. Plant Cell Rep. 2011 Mar;30(3):399-406.
  • Kawakatsu T, Takaiwa F. Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain. Plant Cell Physiol. 2010 Dec;51(12):1964-74.
  • Kawakatsu T, Hirose S, Yasuda H, Takaiwa F. Reducing rice seed storage protein accumulation leads to changes in nutrient quality and storage organelle formation. Plant Physiol. 2010 Dec;154(4):1842-54.
  • Takagi H, Hiroi T, Hirose S, Yang L, Takaiwa F. Rice seed ER-derived protein body as an efficient delivery vehicle for oral tolerogenic peptides. Peptides. 2010 Aug;31(8):1421-5.
  • Oono Y, Wakasa Y, Hirose S, Yang L, Sakuta C, Takaiwa F. Analysis of ER stress in developing rice endosperm accumulating beta-amyloid peptide. Plant Biotechnol J. 2010 Aug;8(6):691-718.
  • Kawakatsu T, Wang S, Wakasa Y, Takaiwa F. Increased lysine content in rice grains by over-accumulation of BiP in the endosperm. Biosci Biotechnol Biochem. 2010;74(12):2529-31.