生物機能利用研究部門

昆虫機能制御ユニット

農業害虫遺伝子の機能や害虫の生物間相互作用の解明に基づいて、環境負荷を少なくした害虫制御に使える基盤技術を開発し、農業被害の軽減に貢献します。

新規の害虫制御剤候補化合物の選抜法を開発します。

図1

トビイロウンカに強いイネが持つ害虫抵抗性遺伝子の構造と機能を明らかにし、活用します。

図2


メンバー

ユニット長
篠田徹郎
主席研究員
小瀧豊美
田中良明
主任研究員
秋月岳
粥川琢巳
研究員
浅野美和

メンバーの主要成果 (プレスリリースした成果など)

原著論文

  • Kayukawa T, Nagamine K, Ito Y, Nishita Y, Ishikawa Y, Shinoda T (2016) Kruppel homolog 1 inhibits insect metamorphosis via direct transcriptional repression of Broad-complex, a pupal specifier gene The Journal of Biological Chemistry 291:1751-1762
  • Tanaka S, Saeki S, Nishide Y, Sugahara R and Shiotsuki T (2016) Body-color and behavioral responses to crowding and visual stimuli in the mid-instar nymphs of the desert locust, Schistocerca gregaria (Orthoptera: Acrididae) Entomol. Sci.
  • Sugahara R, Minaba M, Jouraku A, Kotaki T, Yamamoto T, Shinohara Y, Miyoshi H, Shiotsuki T (2016) Characterization of two adenine nucleotide translocase paralogues in the stink bug, Plautia stali J. Pestic. Sci. 41, 44-48
  • Yamamoto K, Higashiura A, Suzuki M, Shiotsuki T, Sugahara R, Fujii T and Nakagawa A (2016) Structural characterization of aldo-keto reductase (AKR2E5) from the silkworm Bombyx mori Biochem Biophys. Res. Comm., 474, 104-110 (2016).
  • Sugahara R, Tanaka S, Jouraku A, Shiotsuki T (2016) Functional characterization of the corazonin-encoding gene in phase polyphenism of the migratory locust, Locusta migratoria (Orthoptera: Acrididae) Applied Entomology and Zoology 51(2):225-232
  • Osanai-Futahashi M, Tatematsu K, Futahashi R, Narukawa J, Takasu Y, Kayukawa T, Shinoda T, Ishige T, Yajima S, Tamura T, Yamamoto K, Sezutsu H (2016) Positional cloning of a Bombyx pink-eyed white egg locus reveals the major role of cardinal in ommochrome synthesis Heredity 116(2):135-145
  • Nakai M, Kinjo H, Takatsuka J, Shiotsuki T, Kamita S.G, Kunimi Y (2016) Entomopoxvirus infection induces changes in both juvenile hormone and ecdysteroid levels in larval Mythimna separata Journal of General Virology97(1):225-232
  • Kotaki T, Kaihara K, Ando Y, Misaki K, Shinada T (2016) Oosorption in the stink bug Plautia stali: role of Juvenile hormone in the induction of oosorption Physiological Entomology 41(2):127-131
  • Vea I.M, Tanaka S, Shiotsuki T, Jouraku A, Tanaka T, Minakuchi C (2016) Differential juvenile hormone variations in scale insect extreme sexual dimorphism PLoS ONE 11(2):e0149459
  • Kayukawa T, Shinoda T (2015) Functional characterization of two paralogous JH receptors, methoprene-tolerant 1 and 2, in the silkworm, Bombyx mori (Lepidoptera: Bombycidae) Applied Entomology and Zoology 50(3):383-391
  • Daimon T, Uchibori M, Nakao H, Sezutsu H, Shinoda T (2015) Knockout silkworms reveal a dispensable role for juvenile hormones in holometabolous life cycle Proceedings of the National Academy of Sciences of the United States of America 112(31):E4226-E4235
  • Enya S, Daimon T, Igarashi F, Kataoka H, Uchibori M, Sezutsu H, Shinoda T, Niwa R (2015) The silkworm glutathione S-transferase gene noppera-bo is required for ecdysteroid biosynthesis and larval development Insect Biochemistry and Molecular Biology 61:1-7
  • Komura-Kawa T, Hirota K, Shimada-Niwa Y, Yamauchi R, Shimell MJ, Shinoda T, Fukamizu A, O'Connor M.B, Niwa R (2015) The Drosophila zinc finger transcription factor ouija board controls ecdysteroid biosynthesis through specific regulation of spookier PLoS Genetics 11(12):e1005712
  • Minakuchi C, Ishii F, Washidu Y, Ichikawa A, Tanaka T, Miura K, Shinoda T (2015) Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum Journal of Insect Physiology 80:61-70
  • Suga T, Asami Y, Hashimoto S, Nonaka K, Iwatsuki M, Nakashima T, Sugahara R, Shiotsuki T, Yamamoto T, Shinohara Y, Ichimaru N, Murai M, Miyoshi H, ?mura S, Shiomi K (2015) Ascosteroside C, a new mitochondrial respiration inhibitor discovered by pesticidal screening using recombinant Saccharomyces cerevisiae The Journal of Antibiotics 68(10):649-652
  • Suga T, Asami Y, Hashimoto S, Nonaka K, Iwatsuki M, Nakashima T, Watanabe Y, Sugahara R, Shiotsuki T, Yamamoto T, Shinohara Y, Ichimaru N, Murai M, Miyoshi H, ?mura S, Shiomi K (2015) Trichopolyn VI: a new peptaibol insecticidal compound discovered using a recombinant Saccharomyces cerevisiae screening system The Journal of General and Applied Microbiology 61(3):82-87
  • Sugahara R, Saeki S, Jouraku A, Shiotsuki T, Tanaka S (2015) Knockdown of the corazonin gene reveals its critical role in the control of gregarious characteristics in the desert locust Journal of Insect Physiology 79:80-87
  • Sugimoto T.N, Kayukawa T, Matsuo T, Tsuchida T, Ishikawa Y (2015) A short, high-temperature treatment of host larvae to analyze Wolbachia-host interactions in the moth Ostrinia scapulalis Journal of Insect Physiology 81:48-51
  • Matsumoto H, Ueno C, Nakamura Y, Kinjoh T, Ito Y, Shimura S, Noda H, Imanishi S, Mita K, Fujiwara H, Hiruma K, Shinoda T, Kamimura M (2015) Identification of two juvenile hormone inducible transcription factors from the silkworm, Bombyx mori Journal of Insect Physiology 80:31-41
  • Toyota K, Miyakawa H, Hiruta C, Furuta K, Ogino Y, Shinoda T, Tatarazako N, Miyagawa S, Shaw J.R, Iguchi T (2015) Methyl farnesoate synthesis is necessary for the environmental sex determination in the water flea Daphnia pulex Journal of Insect Physiology 80:22-30
  • Sugimoto T.N, Kayukawa T, Shinoda T, Ishikawa Y, Tsuchida T (2015) Misdirection of dosage compensation underlies bidirectional sex-specific death in Wolbachia-infected Ostrinia scapulalis Insect Biochemistry and Molecular Biology 66:72-76
  • Yamamoto K, Higashiura A, Hossain T, Yamada N, Shiotsuki T, Nakagawa A (2015) Structural characterization of the catalytic site of a Nilaparvata lugens delta-class glutathione transferase Archives of Biochemistry and Biophysics 566:36-42
  • Kayukawa T, Murata M, Kobayashi I, Muramatsu D, Okada C, Uchino K, Sezutsu H, Kiuchi M, Tamura T, Hiruma K, Ishikawa Y, Shinoda T (2014) Hormonal regulation and developmental role of Kruppel homolog 1, a repressor of metamorphosis, in the silkworm Bombyx mori Developmental Biology388(1):48-56
  • Enya S, Ameku T, Igarashi F, Iga M, Kataoka H, Shinoda T, Niwa R (2014) A Halloween gene noppera-bo encodes a glutathione S-transferase essential for ecdysteroid biosynthesis via regulating the behaviour of cholesterol in Drosophila Scientific Reports 4:6586
  • Kamimura M, Matsumoto H, Kiuchi M, Ito Y, Fujiwara H, Shinoda T (2014) Development of a cell-based assay for ecdysteroid quantification using an early ecdysteroid-inducible gene promoter Applied Entomology and Zoology 49(3):443-452
  • Lozano J, Kayukawa T, Shinoda T, Belles X (2014) A role for Taiman in insect metamorphosis PLoS Genetics 10(10):e1004769
  • Nagamine K, Kayukawa T, Hoshizaki S, Matsuo T, Shinoda T, Ishikawa Y (2014) Cloning, phylogeny, and expression analysis of the Broad-Complex gene in the longicorn beetle Psacothea hilaris SpringerPlus 3:539
  • Sugahara R, Jouraku A, Nakakura T, Kusakabe T, Yamamoto T, Shinohara Y, Miyoshi H, Shiotsuki T (2015) Two adenine nucleotide translocase paralogues involved in cell proliferation and spermatogenesis in the silkworm Bombyx mori PLoS ONE 10(3):e0119429
  • Sugahara R, Mon H, Lee J.M, Shiotsuki T, Kusakabe T (2014) Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells FEBS Letters 588(21):3959-3963
  • Tsubota T, Uchino K, Suzuki T.K, Tanaka H, Kayukawa T, Shinoda T, Sezutsu H (2014) Identification of a novel strong and ubiquitous promoter/enhancer in the silkworm Bombyx mori G3: Genes, Genomes, Genetics 4(7):1347-1357
  • 上原拓也, 山口照美, 小滝豊美, 霜田政美 (2014) オープンフィールド試験はチャバネアオカメムシPlautia stali(Scott)の走光性評価に有効である 日本応用動物昆虫学会誌 58(1):36-38
  • Smykal V, Daimon T, Kayukawa T, Takaki K, Shinoda T, Jindra M (2014) Importance of juvenile hormone signaling arises with competence of insect larvae to metamorphose Developmental Biology390(2):221-230
  • Tanaka Y, Suetsugu Y, Yamamoto K, Noda H, Shinoda T (2014) Transcriptome analysis of neuroPeptides and G-protein coupled receptors (GPCRs) for neuroPeptides in the brown planthopper Nilaparvata lugens Peptides 53:125-133
  • Kayukawa T, Tateishi K, Shinoda T (2013) Establishment of a versatile cell line for juvenile hormone signaling analysis in Tribolium castaneum Scientific Reports 3:1570
  • Furuta K, Ichikawa A, Murata M, Kuwano E, Shinoda T, Shiotsuki T (2013) Determination by LC-MS of juvenile hormone titers in hemolymph of the silkworm, Bombyx mori Bioscience, Biotechnology and Biochemistry 77(5):988-991
  • Tabuchi M, Sakurai T, Mitsuno H, Namiki S, Minegishi R, Shiotsuki T, Uchino K, Sezutsu H, Tamura T, Haupt S.S, Nakatani K, Kanzaki R (2013) Pheromone responsiveness threshold depends on temporal integration by antennal lobe projection neurons Proceedings of the National Academy of Sciences of the United States of America 110(38):15455-15460
  • Tanaka A, Kuga Y, Tanaka Y, Goto S.G, Numata H, Shiga S (2013) Effects of ablation of the pars intercerebralis on ecdysteroid quantities and yolk protein expression in the blowfly Protophormia terraenovae Physiological Entomology 38(3):192-201
  • Fujimoto Z, Suzuki R, Shiotsuki T, Tsuchiya W, Tase A, Momma M, Yamazaki T (2013) Crystal structure of silkworm Bombyx mori JHBP in complex with 2-Methyl-2,4-pentanediol: Plasticity of JH-binding pocket and ligand-induced conformational change of the second cavity in JHBP PLoS ONE 8(2):e56261
  • Augustin J.M, Drok S, Shinoda T, Sanmiya K, Kvist Nielsen J, Khakimov B, Olsen C.E, Hansen E.H, Kuzina V, Ekstrom C.T, Hauser T.P, Bak S (2012) UDP-glycosyltransferases from the UGT73C subfamily in Barbarea vulgaris catalyse sapogenin 3-O-glucosylation in saponin-mediated insect resistance Plant Physiology 160(4):1881-1895
  • Daimon T, Fujii T, Fujii T, Yokoyama T, Katsuma S, Shinoda T, Shimada T, Ishikawa Y (2012) Reinvestigation of the sex pheromone of the wild silkmoth Bombyx mandarina: The effects of bombykal and bombykyl acetate Journal of Chemical Ecology 38(8):1031-1035
  • Kayukawa T, Minakuchi C, Namiki T, Togawa T, Yoshiyama M, Kamimura M, Mita K, Imanishi S, Kiuchi M, Ishikawa Y, Shinoda T (2012) Transcriptional regulation of juvenile hormone-mediated induction of Kruppel homolog 1, a repressor of insect metamorphosis Proceedings of the National Academy of Sciences of the United States of America 109(29):11729-11734
  • Osanai-Futahashi M, Tatematsu K, Yamamoto K, Narukawa J, Uchino K, Kayukawa T, Shinoda T, Banno Y, Tamura T, Sezutsu H (2012) Identification of the Bombyx red egg gene reveals involvement of a novel transporter family gene in late steps of the insect ommochrome biosynthesis pathway The Journal of Biological Chemistry 287(21):17706-17714
  • Daimon T, Kozaki T, Niwa R, Kobayashi I, Furuta K, Namiki T, Uchino K, Banno Y, Katsuma S, Tamura T, Mita K, Sezutsu H, Nakayama M, Itoyama K, Shimada T, Shinoda T (2012) Precocious metamorphosis in the juvenile hormone-deficient mutant of the silkworm, Bombyx mori PLoS Genetics 8(3):e1002486
  • Ishikawa A, Ogawa K, Gotoh H, Walsh T.K, Tagu D, Brisson J.A, Rispe C, S Jaubert-Possamai S, Kanbe T, Tsubota T, Shiotsuki T, Miura T (2012) Juvenile hormone titre and related gene expression during the change of reproductive modes in the pea aphid Insect Molecular Biology 21(1):49-60
  • Kaihara K, Kotaki T, Numata H, Ohfune Y, Shinada T (2012) Structure-activity relationship of novel juvenile hormone, JHSB3, isolated from the stink bug, Plautia stali Tetrahedron 68(1):106-113

農業生物資源研究所主な研究成果(第III期中期計画中の成果)

  • 『幼若ホルモン』フリーのカイコを作出:新規害虫制御剤の開発が加速(2016) 大門高明,内堀美和,中尾肇,瀬筒秀樹,篠田徹郎
  • 昆虫遺伝子の機能解析に有効なコクヌストモドキ培養細胞株の樹立(2014)粥川琢巳,立石剣,篠田徹郎
  • 幼若ホルモンによる変態抑制遺伝子の発現誘導機構の解明とその利用(2013)粥川琢巳,水口智江可,並木俊樹,外川徹,芳山三千代,神村学,三田和英,今西重雄,木内信,石川幸男,篠田徹郎
  • カイコの「2眠蚕」変異体で早熟変態が起きる原因を解明(2012)大門高明,古崎利紀,古田健次郎,並木俊樹,瀬筒秀樹,小林功,内野恵郎,三田和英,田村俊樹,嶋田透,勝間進,丹羽隆介,伴野豊,糸山享,中山真義,篠田徹郎
  • 昆虫幼若ホルモンの輸送メカニズムの解明(2012)鈴木倫太郎,藤本瑞,塩月孝博,土屋渉,門間充,多勢祥,宮澤光博,山崎俊正

特許

  • 篠田徹郎・水口智江可・神村 学・今西 重雄:幼若ホルモン応答エレメント(特許第5754681号)(2015)

プレスリリース

  • 「お化け」遺伝子を呼び出す「こっくりさん」タンパク質の発見
    ~昆虫のステロイドホルモン生合成に関わる新知見~(2015)
  • 幼若ホルモンがサナギ化を抑えるメカニズムを解明(2015)
  • 『幼若ホルモン』フリーのカイコを作出(2015)
  • 細胞内コレステロールの挙動調節に必須の新しい遺伝子「ノッペラボー」を発見(2014)
  • 昆虫の『変身』を抑える遺伝子のスイッチのしくみを世界で初めて解明
    - 狙いの害虫にのみ作用する薬剤の開発に期待 -(2012)
  • カイコの蛹への変態を抑制する遺伝子を発見
    - 害虫の脱皮・変態を制御する新しい薬剤の開発が期待 -(2012)
  • 世界初、昆虫幼若ホルモンの輸送メカニズムを解明 -害虫だけに効く農薬開発に期待-(2012)