生物機能利用研究部門

動物生体防御ユニット

役割

家畜の生産コスト削減のために、動物の生体防御(免疫)機能を中心とした生理機能について、ゲノムや細胞機能の観点からの解明に取り組むとともに、抗体等の免疫系分子を活用した新素材の開発に関わる研究を行っています。

主な研究テーマ

1 免疫系等の生理機能の機能解析

家畜の生産性を損なう最も大きな原因である感染症の克服のため、病原体感染に対する免疫応答の誘導等、動物の生体防御機能等の生理機能について、モデル動物や家畜を用いて、ゲノミクス・細胞生物学的手法により詳細に解析するとともに、疾患感受性の宿主(動物)側の要因を家畜やモデル動物(マウス)の系を用いて明らかにすることを目指しています。特に、マクロファージ等の自然免疫系細胞の機能の解析や、ヒトやマウス等、免疫学的な知見が集積している動物と比較した際の家畜に特徴的な免疫機構の解明にも取り組んでいます。

【豚の血液由来マクロファージの増殖系の確立】
病原体感染の最前線ではたらく自然免疫細胞の一種であるマクロファージを、豚において血液から単離・増殖させる手法を確立しました。これにより、豚の自然免疫応答について詳細な解析が可能となるとともに、異なる遺伝的背景を有する豚の免疫系のはたらき方の違いを、豚を生かしたまま解析することができるようになりました。

主な研究テーマ

2 遺伝子多様性の免疫能・抗病性等の生理機能に与える影響の探索

家畜感染症の克服のためには、ワクチンや治療薬の開発だけでなく、遺伝的な疾病抵抗性を向上させることが重要です。感染症への抵抗性の高い家畜の作出のために、遺伝子やその他のゲノム領域の多様性と、免疫等の生理機能や、疾患そのものへの感受性・抵抗性との関連について研究を行っています。パターン認識受容体遺伝子をはじめとする免疫系遺伝子中の多型の検索と、検出された多型の病原体認識に与える影響や疾患感受性との関連の解析、あるいは家畜やモデル動物(マウス)を用いたゲノム領域と疾患との関連の解明等に取り組んでいます。

【免疫系遺伝子の多様性と感染症抵抗性の関連】
病原体の認識に関わるパターン認識受容体等の免疫系遺伝子では、豚の集団内に多くの遺伝子配列の違い(多型) が見られ、その中には病原体の認識に影響を与えるものがあります。病原体への応答に影響を与える多型のさらなる検出を進めるとともに、細胞レベル、あるいは豚個体のレベルで疾患への抵抗性・感受性にどのような影響があるのかを解析し、抗病性に優れた豚品種作出に寄与することを目指します。

主な研究テーマ

3 免疫系遺伝子を用いた新素材開発

極めて強い特異的結合活性を持つ抗体は、疾病診断・創薬やタンパク質機能解析などの用途に幅広い産業利用が期待される一方で、製造コストの高さが課題となっています。抗体の産業利用を促進するために、全く新しい観点から抗体活性を有する新素材を安価に大量に生産する必要があります。そこで、カイコ等での組換え抗体産生系により、加工性に優れたシルクの特性を活用するなどにより、病原体の検査や除去に役立つツールの開発等、有用な新素材の開発のための研究を進めています。

【抗体の機能を有する新規絹タンパク質素材の開発】
一本鎖化した抗体の一部分(scFv)を、絹糸の構成タンパク質であるフィブロインと結合したかたちで生産するカイコを、遺伝子組み換え技術により作出し、その繭から抗体活性を持つ「アフィニティーシルク」を抽出します。「アフィニティーシルク」は粉末やフィルム等、様々な形状への加工が可能であり、物質への特異的な結合活性を生かした様々な素材への応用が期待されます。


メンバー

ユニット長
上西 博英
主席研究員
須藤 淳一
主席研究員
竹之内 敬人
上級研究員
佐藤 充
研究員
新開 浩樹

メンバーの主要成果 (プレスリリースした成果など)

  • 「細胞内タンパク質の特定領域の働きを詳細に評価できる新しい技術の開発に成功-細胞内タンパク質の機能解析の加速化に貢献-」(2013年度)
  • 「ブタのゲノム及び遺伝子配列の解読に成功-ブタの品種改良の加速化に期待-」(2012年度)

(原著論文) (過去5年以内に発表したもの)

  • Ando A, Shigenari A, Kojima-Shibata C, Nakajoh M, Suzuki K, Kitagawa H, Shiina T, Inoko H, Uenishi H (2016) Association of swine leukocyte antigen class II haplotypes and immune-related traits in a swine line selected for resistance to mycoplasmal pneumonia Comparative Immunology, Microbiology and Infectious Diseases 48:33-40
  • Iwamaru Y, Kitani H, Okada H, Takenouchi T, Shimizu Y, Imamura M, Miyazawa K, Murayama Y, Hoover E.A, Yokoyama T (2016) Proximity of SCG10 and prion protein in membrane rafts Journal of Neurochemistry 136(6):1204-1218
  • Sato T, Okamura T, Kojima-Shibata C, Kadowaki H, Suzuki E, Uenishi H, Suzuki K (2016) Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs Animal Science Journal 87(4):477-483
  • Shinkai H, Toki D, Okumura N, Takenouchi T, Kitani H, Uenishi H (2016) Polymorphisms of the immune-modulating receptor dectin-1 in pigs: their functional influence and distribution in pig populations Immunogenetics 68(4):275-284
  • Sugama S, Sekiyama K, Kodama T, Takamatsu Y, Takenouchi T, Hashimoto M, Bruno C, Kakinuma Y (2016) Chronic restraint stress triggers the dopaminergic and noradrenergic neurodegeneration: possible role of chronic stress for the onset of Parkinson's disease Brain, Behavior, and Immunity 51:39-46
  • Suzuki R, Saito K, Matsuda M, Sato M, Kanegae Y, Shi G, Watashi K, Aizaki H, Chiba J, Saito I, Wakita T, Suzuki T (2016) Single-domain intrabodies against hepatitis C virus core inhibit viral propagation and core-induced NFκB activation Journal of General Virology 97(4):887-892
  • Suzuki S, Iwamoto M, Hashimoto M, Suzuki M, Nakai M, Fuchimoto D, Sembon S, Eguchi-Ogawa T, Uenshi H, Onishi A (2016) Generation and characterization of RAG2 knockout pigs as animal model for severe combined immunodeficiency Veterinary Immunology and Immunopathology 178:37-49, 2016.
  • Alvarez B, Escalona Z, Uenishi H, Toki D, Revilla C, Yuste M, Gomez del Moral M, Alonso F, Ezquerra A, Dominguez J (2015) Molecular and functional characterization of porcine siglec-3/CD33 and analysis of its expression in blood and tissues Developmental & Comparative Immunology 51(2):238-250
  • Arakawa A, Okumura N, Taniguchi M, Hayashi T, Hirose K, Fukawa K, Ito T, Matsumoto T, Uenishi H, Mikawa S (2015) Genome-wide association QTL mapping for teat number in a purebred population of Duroc pigs Animal Genetics 46(5):571-575
  • Bergman I.M, Okumura N, Uenishi H, Hammer S.E, Knoll A, Edfors I, Juul-Madsen H.R (2015) Wild boars from Sweden, Austria, the Czech Republic and Japan possess intact mannose-binding lectin 2 (MBL2) genes International Journal of Immunogenetics 42(3):204-207
  • Escalona Z, Alvarez B, Uenishi H, Toki D, Yuste M, Revilla C, Gomez del Moral M, Alonso F, Ezquerra A, Dominguez J (2015) Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues Developmental & Comparative Immunology 48(1):116-123
  • Sakuma C, Sato M, Oshima T, Takenouchi T, Chiba J, Kitani H (2015) Anti-WASP intrabodies inhibit inflammatory responses induced by Toll-like receptors 3, 7, and 9, in macrophages Biochemical and Biophysical Research Communications 458(1):28-33
  • Sakuma C, Sato M, Takenouchi T, Kitani H (2015) Specific binding of the WASP N-terminal domain to Btk is critical for TLR2 signaling in macrophages Molecular Immunology 63(2):328-336
  • Satou K, Suto J (2015) Effect of the Y chromosome on testis weight in mice Journal of Veterinary Medical Science 77(6):753-759
  • Shinkai H, Matsumoto T, Toki D, Okumura N, Terada K, Uenishi H (2015) Porcine NOD1 polymorphisms with impaired ligand recognition and their distribution in pig populations Molecular Immunology 63(2):305-311
  • Suto J (2015) Genetic analysis of litter size in mice Journal of Veterinary Medical Science 77(3):353-358
  • Suto J (2015) Genetic analysis of low survival rate of pups in RR/Sgn inbred mice Journal of Veterinary Medical Science 77(7):809-813
  • Suto J, Satou K (2015) Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD.Cg-Ay Journal of Veterinary Medical Science 77(2):203-210
  • Takenouchi T, Tsukimoto M, Iwamaru Y, Sugama S, Sekiyama K, Sato M, Kojima S, Hashimoto M, Kitani H (2015) Extracellular ATP induces unconventional release of glyceraldehyde-3-phosphate dehydrogenase from microglial cells Immunology Letters 167(2):116-124
  • Toki Y, Takenouchi T, Harada H, Tanuma S, Kitani H, Kojima S, Tsukimoto M (2015) Extracellular ATP induces P2X7 receptor activation in mouse Kupffer cells, leading to release of IL-1β, HMGB1, and PGE2, decreased MHC class I expression and necrotic cell death Biochemical and Biophysical Research Communications 458(4):771-776
  • Escalona Z, Alvarez B, Uenishi H, Toki D, Yuste M, Revilla C, Gomez del Moral M, Alonso F, Ezquerra A, Dominguez J (2014) Molecular characterization and expression of porcine Siglec-5 Developmental & Comparative Immunology 44(1):206-216
  • Ishimaru M, Yusuke N, Tsukimoto M, Harada H, Takenouchi T, Kitani H, Kojima S (2014) Purinergic signaling via P2Y receptors up-mediates IL-6 production by liver macrophages/Kupffer cells The Journal of Toxicological Sciences 39(3):413-423
  • Kitani H, Sakuma C, Takenouchi T, Sato M, Yoshioka M, Yamanaka N (2014) Establishment of c-myc-immortalized Kupffer cell line from a C57BL/6 mouse strain Results in Immunology 4:68-74
  • Kitani H, Yoshioka M, Takenouchi T, Sato M, Yamanaka N (2014) Characterization of the liver-macrophages isolated from a mixed primary culture of neonatal swine hepatocytes Results in Immunology 4:1-7
  • Kojima S, Negishi Y, Tsukimoto M, Takenouchi T, Kitani H, Takeda K (2014) Purinergic signaling via P2X7 receptor mediates IL-1β production in Kupffer cells exposed to silica nanoparticle Toxicology 321(3):13-20
  • 溝呂木聖子, 矢ヶ部陽子, 芝原友幸, 秋庭正人, 楠本正博, 佐久間晶子, 中條満, 新開浩樹, 上西博英, 宗田吉広 (2014) HPCD子豚へのSalmonella enterica serovar Choleraesuis実験感染;病態の再現とTLR5-塩基多型(C1205T)の影響検証の試み 日本豚病研究会報 63:6-11
  • Morozumi T, Iseki H, Toki D, Takagi M, Tsunemitsu H, Uenishi H (2014) Concise and broadly applicable method for determining the genomic sequences of North-American-type porcine reproductive and respiratory syndrome viruses in various clusters Journal of Veterinary Medical Science 76(9):1249-1255
  • Sasaki K, Tungtrakoolsub P, Morozumi T, Uenishi H, Kawahara M, Watanabe T (2014) A single nucleotide polymorphism of porcine MX2 gene provides antiviral activity against vesicular stomatitis virus Immunogenetics 66(1):25-32
  • Sato M, Kojima K, Sakuma C, Murakami M, Tamada Y, Kitani H (2014) Production of scFv-conjugated affinity silk film and its application to a novel enzyme-linked immunosorbent assay Scientific Reports 4:4080
  • Sekiyama K, Waragai M, Akatsu H, Sugama S, Takenouchi T, Takamatsu Y, Fujita M, Sekigawa A, Rockenstein E, Inoue S, La Spada A.R, Masliah E, Hashimoto M (2014) Disease modifying effect of adiponectin in model of α-synucleinopathies Annals of Clinical and Translational Neurology 1(7):479-489
  • Suto J, Satou K (2014) Effect of the Y chromosome on plasma high-density lipoprotein-cholesterol levels in Y-chromosome-consomic mouse strains BMC Research Notes 7:393
  • Takenouchi T, Suzuki S, Shinkai H, Tsukimoto M, Sato M, Uenishi H, Kitani H (2014) Extracellular ATP does not induce P2X7 receptor-dependent responses in cultured renal- and liver-derived swine macrophages Results in Immunology 4:62-67
  • Bergman I-M, Sandholm K, Ekdahl K.N, Okumura N, Uenishi H, Guldbrandtsen B, Essler S.E, Knoll A, Heegaard P.M.H, Edfors I, Juul-Madsen H.R (2013) MBL1 genotypes in wild boar populations from Sweden, Austria, the Czech Republic, and Japan International Journal of Immunogenetics 40(2):131-139
  • Dawson H.D, Loveland J.E, Pascal G, Gilbert J.GR, Uenishi H, Mann K.M, Sang Y, Zhang J, Carvalho-Silva D, Hunt T, Hardy M, Hu Z, Zhao S-H, Anselmo A, Shinkai H, Chen C, Badaoui B, Berman D, Amid C, Kay M, Lloyd D, Snow C, Morozumi T, Cheng R.P-Y, Bystrom M, Kapetanovic R, Schwartz J.C, Kataria R, Astley M, Fritz E, Steward C, Thomas M, Wilming L, Toki D, Archibald A.L, Bed'Hom B, Beraldi D, Huang T-H, Ait-Ali T, Blecha F, Botti S, Freeman T.C, Giuffra E, Hume D.A, Lunney J.K, Murtaugh M.P, Reecy J.M, Harrow J.L, Rogel-Gaillard C, Tuggle C.K (2013) Structural and functional annotation of the porcine immunome BMC Genomics 14:332
  • Iwamaru Y, Takenouchi T, Imamura M, Shimizu Y, Miyazawa K, Mohri S, Yokoyama T, Kitani H (2013) Prion replication elicits cytopathic changes in differentiated neurosphere cultures Journal of Virology 87(15):8745-8755
  • Moreno S, Alvarez B, Martinez P, Uenishi H, Revilla C, Ezquerra A, Alonso F, Dominguez J (2013) Analysis of chemokine receptor CCR7 expression on porcine blood T lymphocytes using a CCL19-Fc fusion protein Developmental & Comparative Immunology 39(3): 207-213
  • Nakagaki T, Satoh K, Ishibashi D, Fuse T, Sano K, Kamatari Y.O, Kuwata K, Shigematsu K, Iwamaru Y, Takenouchi T, Kitani H, Nishida N, Atarashi R (2013) FK506 reduces abnormal prion protein through the activation of autolysosomal degradation and prolongs survival in prion-infected mice Autophagy 9(9):1386-1394
  • Okumura N, Matsumoto T, Hayashi T, Hirose K, Fukawa K, Itou T, Uenishi H, Mikawa S, Awata T (2013) Genomic regions affecting backfat thickness and cannon bone circumference identified by genome-wide association study in a Duroc pig population Animal Genetics 44(4):454-457
  • Sato M, Sawahata R, Sakuma C, Takenouchi T, Kitani H (2013) Single domain intrabodies against WASP inhibit TCR-induced immune responses in transgenic mice T cells Scientific Reports 3:3003
  • Sugama S, Takenouchi T, Fujita M, Kitani H, Conti B, Hashimoto M (2013) Corticosteroids limit microglial activation occurring during acute stress Neuroscience 232(1):13-20
  • Suto J (2013) QTL mapping of genes controlling plasma insulin and leptin concentrations: Metabolic effect of obesity QTLs identified in an F2 intercross between C57BL/6J and DDD.Cg-Ay inbred mice Journal of Veterinary Medical Science 75(7):895-907
  • Suto J (2013) Y chromosome of the inbred mouse KK/Ta strain is associated with reduced body size in Y-consomic strains BMC Research Notes 6:64
  • Suto J, Satou K (2013) Genetic background (DDD/Sgn versus C57BL/6J) strongly influences postnatal growth of male mice carrying the Ay allele at the agouti locus: identification of quantitative trait loci associated with diabetes and body weight loss BMC Genetics 14:35
  • Eguchi-Ogawa T, Toki D, Wertz N, Butler J.E, Uenishi H (2012) Structure of the genomic sequence comprising the immunoglobulin heavy constant (IGHC) genes from Sus scrofa Molecular Immunology 52(3-4):97-107
  • Groenen M.A.M, Archibald A.L, Uenishi H, Tuggle C.K, Takeuchi Y, Rothschild M.F, Rogel-Gaillard C, Park C, Milan D, Megens H-J, Li S, Larkin D.M, Kim H, Frantz L.A.F, Caccamo M, Ahn H, Aken B.L, Anselmo A, Anthon C, Auvil L, Badaoui B, Beattie C.W, Bendixen C, Berman D, Blecha F, Blomberg J, Bolund L, Bosse M, Botti S, Bujie Z, Bystrom M, Capitanu B, Carvalho-Silva D, Chardon P, Chen C, Cheng R, Choi S-H, Chow W, Clark R.C, Clee C, Crooijmans R.P.M.A, Dawson H.D, Dehais P, De Sapio F, Dibbits B, Drou N, Du Z-Q, Eversole K, Fadista J, Fairley S, Faraut T, Faulkner G.J, Fowler K.E, Fredholm M, Fritz E, Gilbert J.G.R, Giuffra E, Gorodkin J, Griffin D.K, Harrow J.L, Hayward A, Howe K, Hu Z-L, Humphray S.J, Hunt T, Hornshoj H, Jeon J-T, Jern P, Jones M, Jurka J, Kanamori H, Kapetanovic R, Kim J, Kim J-H, Kim K-W, Kim T-H, Larson G, Lee K, Lee K-T, Leggett R, Lewin H.A, Li Y, Liu W, Loveland J.E, Lu Y, Lunney J.K, Ma J, Madsen O, Mann K, Matthews L, McLaren S, Morozumi T, Murtaugh M.P, Narayan J, Truong Nguyen D, Ni P, Oh S-J, Onteru S, Panitz F, Park E-W, Park H-S, Pascal G, Paudel Y, Perez-Enciso M, Ramirez-Gonzalez R, Reecy J.M, Rodriguez-Zas S, Rohrer G.A, Rund L, Sang Y, Schachtschneider K, Schraiber J.G, Schwartz J, Scobie L, Scott C, Searle S, Servin B, Southey B.R, Sperber G, Stadler P, Sweedler J.V, Tafer H, Thomsen B, Wali R, Wang J, Wang J, White S, Xu X, Yerle M, Zhang G, Zhang J, Zhang J, Zhao S, Rogers J, Churcher C, Schook L.B (2012) Analyses of pig genomes provide insight into porcine demography and evolution Nature 491(7424):393-398
  • Hattori F, Ohshima Y, Seki S, Tsukimoto M, Sato M, Takenouchi T, Suzuki A, Takai E, Kitani H, Harada H, Kojima S (2012) Feasibility study of B16 melanoma therapy using oxidized ATP to target purinergic receptor P2X7 European Journal of Pharmacology 695(1-3):20-26
  • Imajo K, Fujita K, Yoneda M, Nozaki Y, Ogawa Y, Shinohara Y, Kato S, Mawatari H, Shibata W, Kitani H, Ikejima K, Kirikoshi H, Nakajima N, Saito S, Maeyama S, Watanabe S, Wada K, Nakajima A (2012) Hyperresponsivity to low-dose endotoxin during progression to nonalcoholic steatohepatitis is regulated by leptin-mediated signaling Cell Metabolism 16(1):44-54
  • Iwamaru Y, Takenouchi T, Murayama Y, Okada H, Imamura M, Shimizu Y, Hashimoto M, Mohri S, Yokoyama T, Kitani H (2012) Anti-prion activity of Brilliant Blue G PLoS ONE 7(5):e37896
  • Kawano A, Tsukimoto M, Mori D, Noguchi T, Harada H, Takenouchi T, Kitani H, Kojima S (2012) Regulation of P2X7-dependent inflammatory functions by P2X4 receptor in mouse macrophages Biochemical and Biophysical Research Communications 420(1):102-107
  • Kawano A, Tsukimoto M, Noguchi T, Hotta N, Harada H, Takenouchi T, Kitani H, Kojima S (2012) Involvement of P2X4 receptor in P2X7 receptor-dependent cell death of mouse macrophages Biochemical and Biophysical Research Communications 419(2):374-380
  • Kita Y.F, Ando A, Tanaka K, Suzuki S, Ozaki Y, Uenishi H, Inoko H, Kulski J.K, Shiina T (2012) Application of high-resolution, massively parallel pyrosequencing for estimation of haplotypes and gene expression levels of swine leukocyte antigen (SLA) class I genes Immunogenetics 64(3):187-199
  • Matsumoto T, Nakajima I, Eguchi-Ogawa T, Nagamura Y, Hamasima N, Uenishi H (2012) Changes in gene expression in a porcine preadipocyte cell line during differentiation Animal Genetics 43(5):535-544
  • Matsumoto T, Okumura N, Uenishi H, Hayashi T, Hamasima N, Awata T (2012) Population structure of pigs determined by single nucleotide polymorphisms observed in assembled expressed sequence tags Animal Science Journal 83(1):14-22
  • Matsumoto T, Okumura N, Uenishi H, Hayashi T, Hamasima N, Awata T (2012) Porcine single nucleotide polymorphism (SNP) development and population structure of pigs assessed by validated SNPs Biochemical Genetics 50(5-6):428-439
  • Muneta Y, Minagawa Y, Kusumoto M, Shinkai H, Uenishi H, Splichal I (2012) Allele-specific primer polymerase chain reaction for a single nucleotide polymorphism (C1205T) of swine Toll-like receptor 5 and comparison of the allelic frequency among several pig breeds in Japan and the Czech Republic Microbiology and Immunology 56(6):385-391
  • Muneta Y, Minagawa Y, Kusumoto M, Shinkai H, Uenishi H, Splichal I (2012) Development of allele-specific primer PCR for a swine TLR2 SNP and comparison of the frequency among several pig breeds of Japan and the Czech Republic Journal of Veterinary Medical Science 74(5):553-559
  • Okamura T, Onodera W, Tayama T, Kadowaki H, Kojima-Shibata C, Suzuki E, Uemoto Y, Mikawa S, Hayashi T, Awata T, Fujishima-Kanaya N, Mikawa A, Uenishi H, Suzuki K (2012) A genome-wide scan for quantitative trait loci affecting respiratory disease and immune capacity in Landrace pigs Animal Genetics 43(6):721-729
  • Sakuma C, Sato M, Takenouchi T, Chiba J, Kitani H (2012) Critical roles of the WASP N-terminal domain and Btk in LPS-induced inflammatory response in macrophages PLoS ONE 7(1):e30351
  • Sakuma C, Sato M, Takenouchi T, Chiba J, Kitani H (2012) Single-chain variable fragment intrabody impairs LPS-induced inflammatory responses by interfering with the interaction between the WASP N-terminal domain and Btk in macrophages Biochemical and Biophysical Research Communications 423(1):164-169
  • Sato M, Kojima K, Sakuma C, Murakami M, Aratani E, Takenouchi T, Tamada Y, Kitani H (2012) Production of scFv-conjugated affinity silk powder by transgenic silkworm technology PLoS ONE 7(4):e34632
  • Sekiyama K, Fujita M, Sekigawa A, Takamatsu Y, Waragai M, Takenouchi T, Sugama S, Hashimoto M (2012) Ibuprofen ameliorates protein aggregation and astrocytic gliosis, but not cognitive dysfunction, in a transgenic mouse expressing dementia with Lewy bodies-linked P123H β-synuclein Neuroscience Letters 515(1):97-101
  • Sekiyama K, Nakai M, Fujita M, Takenouchi T, Waragai M, Wei J, Sekigawa A, Takamatsu Y, Sugama S, Kitani H, Hashimoto M (2012) Theaflavins stimulate autophagic degradation of α-synuclein in neuronal cells Open Journal of Neuroscience 2:1
  • Shinkai H, Arakawa A, Tanaka-Matsuda M, Ide-Okumura H, Terada K, Chikyu M, Kawarasaki T, Ando A, Uenishi H (2012) Genetic variability in swine leukocyte antigen class II and Toll-like receptors affects immune responses to vaccination for bacterial infections in pigs Comparative Immunology, Microbiology and Infectious Diseases 35(6):523-532
  • Shinkai H, Okumura N, Suzuki R, Muneta Y, Uenishi H (2012) Toll-like receptor 4 polymorphism impairing lipopolysaccharide signaling in Sus scrofa, and its restricted distribution among Japanese wild boar populations DNA and Cell Biology 31(4):575-581
  • Suto J (2012) Development of an efficient method for genotyping at the gd locus in NC/Sgn mice based on PCR-RFLP analysis Journal of Veterinary Medical Science 74(8):1003-1006
  • Suto J (2012) Quantitative trait loci that control plasma lipid levels in an F2 intercross between C57BL/6J and DDD.Cg-Ay inbred mouse strains Journal of Veterinary Medical Science 74(4):449-456
  • Suto J (2012) Quantitative trait locus mapping of genes associated with vacuolation in the adrenal X-zone of the DDD/Sgn inbred mouse BMC Genetics 13:95
  • Suto J (2012) Quantitative trait locus mapping of genes that control body length and plasma insulin-like growth factor 1 level in mice BMC Research Notes 5:547
  • Suzuki A, Okumura N, Uehara Y, Uenishi H, Awata T (2012) An identification method for the Tokyo X strain; The pig brand of the Tokyo Metropolitan Agriculture and Forestry Research Center Journal of Animal and Veterinary Advances 11(22):4236-4245
  • Suzuki K, Kobayashi E, Yamashita H, Uenishi H, Churkina I, Plastow G, Hamasima N, Mitsuhashi T (2012) Structural analysis of MHC alleles in an RSV tumour regression chicken using a BAC library Animal Genetics 43(3):348-351
  • Takenouchi T, Iwamaru Y, Imamura M, Fukuhara S, Sugama S, Sato M, Mochizuki N, Hashimoto M, Yokoyama T, Mohri S, Kitani H (2012) Cytochalasin D enhances the accumulation of a protease-resistant form of prion protein in ScN2a cells: involvement of PI3 kinase/Akt signalling pathway Cell Biology International 36(12):1223-1231
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