食品総合研究所

糖質素材ユニット

役割

澱粉産業、製糖産業、糖質素材製造産業等の糖質関連産業は、消費者へ貴重な栄養源・エネルギー源や有用オリゴ糖等の機能性食品素材を供給するのみならず、物理特性、加工適性等に注目した多様な糖質素材を食品加工業者等に提供することにより、我が国の豊かな食生活の実現に多方面から貢献しています。糖質素材ユニットでは、我が国の糖質関連産業の健全な発展に貢献するため、糖質の特性を科学的に解明・評価するとともに、新規糖質素材の製造に資する新たな基盤技術の開発を行っています。また、稲わら等のバイオマスをバイオエタノール等の有用素材へ変換するための技術開発を行っています。

 

主な研究テーマ

糖質素材ユニットでは、主に生物工学技術および糖質工学技術を活用することにより、糖質資源を有用素材へ変換するための新技術の開発、糖質やそれを含む食品の機能を評価する新技術の開発などに取り組んでいます。具体的には、以下の三本柱に注目した研究を実施しています。

  • 新機能を有する糖質素材の製造技術の開発
  • 糖質素材または食品中の糖質の特性解明とその評価技術の開発
  • 稲わら等のバイオマスの有用物質への変換のための技術の開発

消化モデルによる食品の血糖応答性測定法
また、当ユニットでは、上記三本柱に関連する共同研究を積極的にお受けすることにより、我が国の糖質研究および産業の発展に貢献したいと考えています。共同研究の可能性をご検討頂ける大学、企業の方々は是非一回研究室にお越し下さい。また、ユニット長の徳安は徳島大学大学院助教授を併任しており、博士課程学生(一般、社会人、外国人枠)の受け入れが可能です。ご興味をお持ちの方は是非ご一報下さい。

 

メンバー


非常勤職員
田伏美峰(たぶせ みね)
中山浩美(なかやま ひろみ)
宮本惠郷(みやもと よしのり)
耿小嘉(こう しょうか)
吉井のぶ子(よしい のぶこ)

 

主要成果

  • DiSC (direct saccharification of culms) process for bioethanol production from rice straw, Jeung-yil Park, Masakazu Ike, Mitsuhiro Arakane, Riki Shiroma, Yuan Li, Yumiko Arai-Sanoh, Motohiko Kondo, Ken Tokuyasu: Bioresour. Technol., in press (2011)
  • Bioethanol production from rice straw by a sequential use of Saccharomyces cerevisiae and Pichia stipitis with heat inactivation of Saccharomyces cerevisiae cells prior to xylose fermentation, Yuan Li, Jeung-yil Park, Riki Shiroma, Ken Tokuyasu: J. Biosci. Bioeng., in press, (2011)
  • An improved CARV process for bioethanol production from a mixture of sugar beet mash and potato mash. Min-Soo Yun, Jeung-yil Park, Mitsuhiro Arakane, Riki Shiroma, Masakazu Ike, Seiji Tamiya, Hiroyuki Takahashi, Ken Tokuyasu: Biosci. Biotechnol. Biochem., in press, (2011).
  • Low temperature alkali pretreatment for improving enzymatic digestibility of sweet sorghum bagasse for ethanol production, Long Wu, Mitsuhiro Arakane, Masahisa Wada, Tomoya Takai, Mitsuru Gau, Ken Tokuyasu: Bioresour. Technol., 102(7) 4793-4799 (2011)
  • RT-CaCCO process: An improved CaCCO process for rice straw by its incorporation with a step of lime pretreatment at room temperature.Riki Shiroma, Jeung-yil Park, Muhammad Imran Al-Haq, Mitsuhiro Arakane, Masakazu Ike, Ken Tokuyasu: Bioresour. Technol., 102, 2943-2949, (2011)
  • ミキサーの消費電力測定による米粉パン生地のミキシング特性の解析, 與座宏一, 松木順子, 岡留博司, 徳安健: 食総研報, 75,39-44(2011)
  • Bioconversion of L-arabinose and other carbohydrates from plant cell walls to α-glucan by a soil bacterium, Sporosarcina sp. N52. Zilian Zhang, Sathaporn Srichuwong, Tooru Kobayashi, Mitsuhiro Arakane, Jeung-yil Park, Ken Tokuyasu: Bioresour. Technol., 101(24), 9734-9741 (2010)
  • Alkali-aided enzymatic viscosity reduction of sugar beet mash for novel bioethanol production process, Sathaporn Srichuwong, Mitsuhiro Arakane, Maki Fujiwara, Zilian Zhang, Hiroyuki Takahashi, Ken Tokuyasu: Biomass Bioenerg., 34(9) 1336-1341 (2010)
  • A novel lime pretreatment for subsequent bioethanol production from rice straw - Calcium capturing by carbonation (CaCCO) process, Jeung-yil Park, Riki Shiroma, Muhammad Imran Al-Haq, Ying Zhang, Masakazu Ike, Yumiko Arai-Sanoh, Akira Ida, Motohiko Kondo, Ken Tokuyasu: Bioresour. Technol., 101, 6805-6811 (2010)
  • Enzymatic hydrolysis of cellulose I is greatly accelerated via its conversion to the cellulose II hydrate form. Masahisa Wada, Masakazu Ike, Ken Tokuyasu: Polym. Degrad. Stabil., 95, 543-548 (2010)
  • Culm in rice straw as a new carbon source for sugar recovery via enzymatic saccharification, Jeung-yil Park, Mitsuhiro Arakane, Riki Shiroma, Masakazu Ike, Ken Tokuyasu: Biosci. Biotechnol. Biochem., 74, 50-55 (2010)
  • 製粉方法の異なる米粉の特性と製パン性の関係, 與座宏一,松木順子,岡留博司,岡部繭子,鈴木啓太郎,奥西智哉,北村義明,堀金彰,山田純代,松倉潮: 食総研報, 74,37-44(2010)
  • Characterization of starch granules in rice culms for application of rice straw as a feedstock for saccharification, J. Matsuki, J. Park, R. Shiroma, Y. Arai-Sanoh, M. Ida, M. Kondo, K. Motobayashi, K. Tokuyasu: Biosci. Biotechnol. Biochem., 74, 1645-1651 (2010)
  • Cellulase production on glucose-based media by the UV-irradiated mutants of Trichoderma reesei, M. Ike, J.Y. Park, M. Tabuse, K. Tokuyasu: Appl. Microbiol. Biotechnol., 87, 2059-2066 (2010)
  • Simultaneous saccharification and fermentation (SSF) of very high gravity (VHG) potato mash for the production of ethanol. Sathaporn Srichuwong, Maki Fujiwara, Xiaohui Wang, Tomoko Seyama, Riki Shiroma, Mitsuhiro Arakane, Nobuhiro Mukojima, Ken Tokuyasu: Biomass Bioenerg., 33(5) 890-898 (2009)
  • Efficient recovery of glucose and fructose via enzymatic saccharification of rice straw with soft carbohydrates, Jeung-yil Park, Tomoko Seyama, Riki Shiroma, Masakazu Ike, Sathaporn Srichuwong, Kenichi Nagata, Yumiko Arai-Sanoh, Motohiko Kondo, Ken Tokuyasu: Biosci. Biotechnol. Biochem., 73(5):1072-1077 (2009)
  • Pretreatment of microcrystalline cellulose flakes with CaCl2 increases the surface area, and thus improves enzymatic saccharification, Ken Tokuyasu, Mine Tabuse, Junko Matsuki, Koichi Yoza: Carbohydr Res., 343(7):1232-1236 (2008)
  • 米粉利用の現状と課題 --米粉パンについて --,與座宏一, 岡部 繭子, 島 純: 食科工,55, 444-454 (2008)
  • Effects of the barley amo1 and wax genes on starch structure and physicochemical properties, J. Matsuki, T. Yasui, T. Sasaki, M. Fujita, Y. Kitamura: Starch/Starke., 60, 279-285 (2008)
  • Purification and characterization of a chitinase from Amycolatopsis orientalis with N-acetyllactosamine-repeating unit releasing activity, T. Murata, S. Amarume, T. Hattori, S. Tokuyama, K. Tokuyasu, H. Kawagishi, T. Usui, Biochem. Biophys. Res. Commun., 336, 514-20 (2005)
  • Avidin expressed in transgenic rice confers resistance to the stored-product insect pests Tribolium confusum and Sitotroga cerealella, Yoza K, Imamura T, Kramer KJ, Morgan TD, Nakamura S, Akiyama K, Kawasaki S, Takaiwa F, Ohtsubo K: Biosci. Biotechnol. Biochem., 69, 966-71(2005)
  • Subsite structure of the endo-type chitin deacetylase from a Deuteromycete, Colletotrichum lindemuthianum: an investigation using steady-state kinetic and MS, O. Hekmat, K. Tokuyasu, SG. Withers: Biochem. J., 374, 369-380 (2003)
  • Molecular cloning and functional expression of cDNA encoding a cysteine proteinase inhibitor, cystatin, from Job's tears (Coix lacryma-jobi L. var. Ma-yuen Stapf). Yoza K, Nakamura S, Yaguchi M, Haraguchi K, Ohtsubo K: Biosci. Biotechnol. Biochem., 66, 2287-91.(2002)
  • Effects of environmental temperature on structure and gelatinization properties of wheat starch, J. Matsuki, T. Yasui, K. Kohyama, T. Sasaki: Cereal Chem., 80(4), 476-480 (2003)
  • Recognition of chitooligosaccharides and their N-acetyl groups by putative subsites of chitin deacetylase from a deuteromycete, Colletotrichum lindemuthianum; K. Tokuyasu, M. Mitsutomi, I. Yamaguchi, K. Hayashi, Y. Mori: Biochemistry, 39(30), P8837-8843 (2000)
  • Synthesis of a chitosan tetramer derivative,beta-D-GlcNAc-(1-->4)-beta-D-GlcNAc- (1-->4)-beta-D-GlcNAc-(1-->4)-D-GlcN through a partial N-acetylation reaction by chitin deacetylase; K. Tokuyasu, H. Ono, M. Mitsutomi, K. Hayashi, Y. Mori: Carbohydr. Res., 325(3), 211-215 (2000)
  • N-deacetylation of Sinorhizobium meliloti Nod factors increases their stability in the Medicago sativa rhizosphere and decreases their biological activity; C. Staehelin, M. Schultze, K. Tokuyasu, V. Poinsot, J. C. Prome, E. Kondorosi, A. Kondorosi: Mol. Plant Microbe Interact., 13, 72-79 (2000)
  • Production of a recombinant chitin deacetylase in the culture medium of Escherichia coli cell; K. Tokuyasu, S. Kaneko, K. Hayashi, Y. Mori: FEBS Lett., 458, 23-26 (1999)
  • Reverse hydrolysis reaction of chitin deacetylase and enzymatic synthesis of beta-D-GlcNAc-(1-->4)-GlcN from chitobiose; K. Tokuyasu, H. Ono, K. Hayashi, Y. Mori: Carbohydr. Res., 322, P26-31 (1999)
  • Selective N-deacetylation of p-nitrophenyl N,N'-diacetyl-chitobioside and its use to differentiate the action of two types of chitinases; K. Tokuyasu, H. Ono, Y. Kitagawa, K. Hayashi, Y. Mori: Carbohydr. Res., 316, 173-178 (1999)
  • Cloning and expression of chitin deacetylase gene from a Deuteromycete, Colletotrichum lindemuthianum; K. Tokuyasu, M. Ohnishi-Kameyama, K. Hayashi, Y. Mori: J. Biosci. Bioeng., 87, 418-423 (1999)