研究業績

原著論文      総説・著書      特許      受賞


原著論文

Nakata M, Miyashita T, Kimura R, Nakata Y, Takagi H, Kuroda M, Yamaguchi T, Umemoto T, Yamakawa H (2017) MutMapPlus identified novel mutant alleles of a rice starch branching enzyme IIb gene for fine-tuning of cooked rice texture. Plant Biotechnol. J. 15: in press
DOI: 10.1111/pbi.12753


Yamakawa H, Hirai-Kimura R, Nakata Y, Nakata M, Kuroda M, Yamaguchi T (2017) An Activity-Staining Method on Filtration Paper Enables High-Throughput Screening of Temperature-Sensitive and Inactive Mutations of Rice α-Amylase for Improvement of Rice Grain Quality. Plant Cell Physiol. 58: 658-667
DOI: 10.1093/pcp/pcx030


Hakata M, Muramatsu M, Nakamura H, Hara N, Kishimoto M, Iida-Okada K, Kajikawa M, Imai-Toki N, Toki S, Nagamura Y, Yamakawa H, Ichikawa H (2017) Overexpression of TIFY genes promotes plant growth in rice through jasmonate signaling. Biosci. Biotechnol. Biochem. 81: 906-913
DOI: 10.1080/09168451.2016.1274638


Yuki Y, Kurokawa S, Kozuka-Hata H, Tokuhara D, Mejima M, Kuroda M, Oyama M, Nishimaki-Mogami T, Teshima R, Kiyono H (2016) Differential analyses of major allergen proteins in wild-type rice and rice producing a fragment of anti-rotavirus antibody. Regul. Toxicol. Pharmacol. 76: 128-136
DOI: 10.1016/j.yrtph.2016.01.023


Kashima K, Yuki Y, Mejima M, Kurokawa S, Suzuki Y, Minakawa S, Takeyama N, Fukuyama Y, Azegami T, Tanimoto T, Kuroda M, Tamura M, Gomi Y, Kiyono H (2016) Good Manufacturing Practices Production of a purification-free oral cholera vaccine expressed in transgenic rice plants. Plant Cell Rep. 35: 667-679
DOI: 10.1007/s00299-015-1911-9


Takeyama N, Yuki Y, Tokuhara D, Oroku K, Mejima M, Kurokawa S, Kuroda M, Kodama T, Nagai S, Ueda S, Kiyono H (2015) Oral rice-based vaccine induces passive and active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs. Vaccine 33: 5204-5211
DOI: 10.1016/j.vaccine.2015.07.074


Kuroda M, Ikenaga S (2015) Single-tube hydroponics as a novel idea for small-scale production of crop seed in a plant incubator. Biosci. Biotechnol. Biochem. 79: 63-67
DOI: 10.1080/09168451.2014.951026


Mejima M, Kashima K, Kuroda M, Takeyama N, Kurokawa S, Fukuyama Y, Kiyono H, Itoh K, Mitsui T, Yuki Y (2015) Determination of genomic location and structure of the transgenes in marker-free rice-based cholera vaccine by using whole genome resequencing approach. Plant Cell Tiss. Organ Cult. 120: 35-48
DOI: 10.1007/s11240-014-0575-4


Toyomasu T, Usui M, Sugawara C, Kanno Y, Sakai A, Takahashi H, Nakazono M, Kuroda M, Miyamoto K, Morimoto Y, Mitsuhashi W, Okada K, Yamaguchi S, Yamane H (2015) Transcripts of two ent-copalyl diphosphate synthase genes differentially localize in rice plants according to their distinct biological roles. J. Exp. Bot. 66: 369-376
DOI: 10.1093/jxb/eru424


Kashima K, Mejima M, Kurokawa S, Kuroda M, Kiyono H, Yuki Y (2015) Comparative whole-genome analyses of selection marker-free rice-based cholera toxin B-subunit vaccine lines and wild-type lines. BMC Genomics 16: 48
DOI: 10.1186/s12864-015-1285-y


Midorikawa K, Kuroda M, Terauchi K, Hoshi M, Ikenaga S, Ishimaru Y, Abe K, Asakura T (2014) Additional nitrogen fertilization at heading time of rice down-regulates cellulose synthesis in seed endosperm. PLoS One 9: e98738
DOI: 10.1371/journal.pone.0098738


Kurokawa S, Kuroda M, Mejima M, Nakamura R, Takahashi Y, Sagara H, Takeyama N, Satoh S, Kiyono H, Teshima R, Masumura T, Yuki Y (2014) RNAi-mediated suppression of endogenous storage proteins leads to a change in localization of overexpressed cholera toxin B-subunit and the allergen protein RAG2 in rice seeds. Plant Cell Rep. 33: 75-87
DOI: 10.1007/s00299-013-1513-3


Toyomasu T, Usui M, Sugawara C, Otomo K, Hirose Y, Miyao A, Hirochika H, Okada K, Shimizu T, Koga J, Hasegawa M, Chuba M, Kawana Y, Kuroda M, Minami E, Mitsuhashi W, Yamane H (2014) Reverse-genetic approach to verify physiological roles of rice phytoalexins: characterization of a knockdown mutant of OsCPS4 phytoalexin biosynthetic gene in rice. Physiol. Plant. 150: 55-62
DOI: 10.1111/ppl.12066


Abe M, Yuki Y, Kurokawa S, Mejima M, Kuroda M, Park EJ, Scheller J, Nakanishi U, Kiyono H (2014) A rice-based soluble form of a murine TNF-specific llama variable domain of heavy-chain antibody suppresses collagen-induced arthritis in mice. J. Biotechnol. 175: 45-52
DOI: 10.1016/j.jbiotec.2014.02.005


Cabanos C, Kato N, Amari Y, Fujiwara K, Ohno T, Shimizu K, Goto T, Shimada M, Kuroda M, Masuda T, Takaiwa F, Utsumi S, Nagaoka S, Maruyama N (2014) Development of a novel transgenic rice with hypocholesterolemic activity via high-level accumulation of the α' subunit of soybean β-conglycinin. Transgenic Res. 23: 609-620
DOI: 10.1007/s11248-014-9793-0


Yuki Y, Mejima M, Kurokawa S, Hiroiwa T, Takahashi Y, Tokuhara D, Nochi T, Katakai Y, Kuroda M, Takeyama N, Kashima K, Abe M, Chen Y, Nakanishi U, Masumura T, Takeuchi Y, Kozuka-Hata H, Shibata H, Oyama M, Tanaka K, Kiyono H (2013) Induction of toxin-specific neutralizing immunity by molecularly uniform rice-based oral cholera toxin B subunit vaccine without plant-associated sugar modification. Plant Biotechnol. J. 11: 799-808
DOI: 10.1111/pbi.12071


Kurokawa S, Nakamura R, Mejima M, Kozuka-Hata H, Kuroda M, Takeyama N, Oyama M, Satoh S, Kiyono H, Masumura T, Teshima R, Yuki Y (2013) MucoRice-cholera toxin B-subunit, a rice-based oral cholera vaccine, down-regulates the expression of α-amylase/trypsin inhibitor-like protein family as major rice allergens. J Proteome Res. 12: 3372-3382
DOI: 10.1021/pr4002146


Cabanos C, Ekyo A, Amari Y, Kato N, Kuroda M, Nagaoka S, Takaiwa F, Utsumi S, Maruyama N (2013) High-level production of lactostatin, a hypocholesterolemic peptide, in transgenic rice using soybean A1aB1b as carrier. Transgenic Res. 22: 621-629
DOI: 10.1007/s11248-012-9672-5


Tokuhara D, Alvarez B, Mejima M, Hiroiwa T, Takahashi Y, Kurokawa S, Kuroda M, Oyama M, Kozuka-Hata H, Nochi T, Sagara H, Aladin F, Marcotte H, Frenken LG, Iturriza-Gomara M, Kiyono H, Hammarstrom L, Yuki Y (2013) Rice-based oral antibody fragment prophylaxis and therapy against rotavirus infection. J. Clin. Invest. 123: 3829-3838
DOI: 10.1172/JCI70266


Hakata M, Kuroda M, Miyashita T, Yamaguchi T, Kojima M, Sakakibara H, Mitsui T, Yamakawa H (2012) Suppression of α-amylase genes improves quality of rice grain ripened under high temperature. Plant Biotechnol. J. 10: 1110-1117
DOI: 10.1111/j.1467-7652.2012.00741.x


Hakata M, Kuroda M, Ohsumi A, Hirose T, Nakamura H, Muramatsu M, Ichikawa H, Yamakawa H (2012) Overexpression of a rice TIFY gene increases grain size through enhanced accumulation of carbohydrates in the stem. Biosci. Biotechnol. Biochem. 76: 2129-2134
DOI: 10.1271/bbb.120545


Saito Y, Shigemitsu T, Yamasaki R, Sasou A, Goto F, Kishida K, Kuroda M, Tanaka K, Morita S, Satoh S, Masumura T (2012) Formation mechanism of the internal structure of type I protein bodies in rice endosperm: relationship between the localization of prolamin species and the expression of individual genes. Plant J. 70: 1043-1055
DOI: 10.1111/j.1365-313X.2012.04947.x


Shigemitsu T, Ozaki S, Saito Y, Kuroda M, Morita S, Satoh S, Masumura T (2012) Production of human growth hormone in transgenic rice seeds: co-introduction of RNA interference cassette for suppressing the gene expression of endogenous storage proteins. Plant Cell Rep. 31: 539-549
DOI: 10.1007/s00299-011-1191-y


Ishimoto M, Kuroda M, Yoza K, Nishizawa K, Teraishi M, Mizutani N, Ito K, Moriya S (2012) Heterologous expression of corn cystatin in soybean and effect on growth of the stink bug. Biosci. Biotechnol. Biochem. 76: 2142-2145
DOI: 10.1271/bbb.120432


Yuki Y, Mejima M, Kurokawa S, Hiroiwa T, Kong IG, Kuroda M, Takahashi Y, Nochi T, Tokuhara D, Kohda T, Kozaki S, Kiyono H (2012) RNAi suppression of rice endogenous storage proteins enhances the production of rice-based Botulinum neutrotoxin type A vaccine. Vaccine 30: 4160-4166
DOI: 10.1016/j.vaccine.2012.04.064


奥西智哉、宮下香苗、大江翔太郎、萬代悠太、増村威宏、黒田昌治 (2012) 少量試料による米粉生地の膨化測定 日本食品科学工学会誌 59: 473-475
DOI: 10.3136/nskkk.59.473


Ohnishi T, Yoshino M, Yamakawa H, Kinoshita T (2011) The biotron breeding system: A rapid and reliable procedure for genetic studies and breeding in rice. Plant Cell Physiol. 52: 1249-1257
DOI: 10.1093/pcp/pcr066


Yamakawa H, Hakata M (2010) Atlas of rice grain filling-related metabolism under high temperature: joint analysis of metabolome and transcriptome demonstrated inhibition of starch accumulation and induction of amino acid accumulation. Plant Cell Physiol. 51: 795-809
DOI: 10.1093/pcp/pcq034


She KC, Kusano H, Koizumi K, Yamakawa H, Hakata M, Imamura T, Fukuda M, Naito N, Tsurumaki Y, Yaeshima M, Tsuge T, Matsumoto K, Kudoh M, Itoh E, Kikuchi S, Kishimoto N, Yazaki J, Ando T, Yano M, Aoyama T, Sasaki T, Satoh H, Shimada H (2010) A novel factor FLOURY ENDOSPERM 2 is involved in regulation of rice grain size and starch quality. Plant Cell 22: 3280-3294
DOI: 10.1105/tpc.109.070821


Kuroda M, Kimizu M, Mikami C (2010) A simple set of plasmids for the production of transgenic plants. Biosci. Biotechnol. Biochem. 74: 2348-2351
DOI: 10.1271/bbb.100465


Yamaguchi T, Kuroda M, Yamakawa H, Ashizawa T, Hirayae K, Kurimoto L, Shinya T, Shibuya N (2009) Suppression of a phospholipase D gene, OsPLDβ1, activates defense responses and increases disease resistance in rice. Plant Physiol. 150: 308-319
DOI: 10.1104/pp.108.131979


Tamura T, Kuroda M, Oikawa T, Kyozuka J, Terauchi K, Ishimaru Y, Abe K, Asakura T (2009) Signal peptide peptidases are expressed in the shoot apex of rice, localized to the endoplasmic reticulum. Plant Cell Rep. 28:1615-1621
DOI: 10.1007/s00299-009-0760-9


Kiyosaki T, Asakura T, Matsumoto I, Tamura T, Terauchi K, Funaki J, Kuroda M, Misaka T, Abe K (2009) Wheat cysteine proteases triticain alpha, beta and gamma exhibit mutually distinct responses to gibberellin in germinating seeds. J. Plant Physiol. 166: 101-106
DOI: 10.1016/j.jplph.2008.02.006


Yamakawa H, Ebitani T, Terao T (2008) Comparison between locations of QTLs for grain chalkiness and genes responsive to high temperature during grain filling on the rice chromosome map. Breed. Sci. 58: 337-343
DOI: 10.1270/jsbbs.58.337


Yamakawa H, Hirose T, Kuroda M, Yamaguchi T (2007) Comprehensive expression profiling of rice grain filling-related genes under high temperature using DNA microarray. Plant Physiol. 144: 258-277
DOI: 10.1104/pp.107.098665


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総説・著書


山川博幹 (2016) コメの品質に対する温暖化の影響と品種改良の現状 理大科学フォーラム 389: 12-15


Mitsui T, Yamakawa H, Kobata T (2016) Molecular physiological aspects of chalking mechanism in rice grains under high-temperature stress. Plant Prod. Sci. 19: 22-29
DOI: 10.1080/1343943X.2015.1128112


羽方誠、山川博幹 (2014) α-アミラーゼ遺伝子はイネの高温登熟で発生する乳白粒に関与する 応用糖質科学 4(3): 206-208


大西孝幸、吉野みほ子、山川博幹、木下哲 (2014) イネのバイオトロン栽培法の紹介 応用糖質科学 4(3): 215-218


山川博幹、羽方 誠、黒田昌治、宮下朋美、山口武志、小嶋美紀子、榊原 均、三ツ井敏明 (2013) 高温で乳白粒が発生する原因を遺伝子レベルで解明-高温登熟耐性品種の開発に期待- JATAFFジャーナル 1(4): 24-27


山口武志、黒田昌治、山川博幹、羽方 誠 (2013) 植物におけるホスホリパーゼDの生理機能 オレオサイエンス 13(10): 471-476


黒田昌治 (2012) 米を利用した医薬品タンパク質生産および次世代ワクチン開発研究 食品と容器 53: 352-357


山川博幹、羽方 誠 (2011) イネも夏バテをする?/遺伝子レベルで明らかとなった猛暑が米の品質に及ぼす影響 化学と生物 49: 624-629
DOI: 10.1271/kagakutoseibutsu.49.624


Yamakawa H (2011) Omics-based approach for cereal starch biosynthesis: Toward a determination of key factors for quality of rice grain affected by high temperature. J Appl. Glycosci. 58: 35-38
DOI: 10.5458/jag.jag.JAG-2010_019


三ツ井敏明、山川博幹 (2009) イネの高温登熟障害と熱ショック 農林水産技術研究ジャーナル 32: 20-25


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特許


山口武志、黒田昌治、山川博幹 (2015) イネ科植物の高温障害を低減させる方法、ベクター及びイネ科植物 特許第5812386号


黒田昌治 (2010) 種子中のタンパク質含量が低減した植物ならびにその作出法および利用法 特許第4644777号


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受賞


平成25年(2013年)日本植物生理学会論文賞 大西孝幸、吉野美穂子、山川博幹、木下 哲 「The biotron breeding system: A rapid and reliable procedure for genetic studies and breeding in rice」


平成24年(2012年)農林水産研究成果10大トピックス 山川博幹、羽方 誠、三ツ井敏明、榊原 均 「高温で乳白粒が発生する原因を遺伝子レベルで解明-高温登熟耐性品種の開発に期待-」


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