氏名

藤井　毅　（ふじい　たけし）　FUJII, Takeshi

所属・職名

生物生態機能研究領域領域長

現在の研究内容

カタボライト抑制機構はじめ放線菌キチナーゼ遺伝子群の発現制御機構の解析。
放線菌キチナーゼの大量生産株の開発とその応用。
土壌抽出DNAおよびRNAを用いた環境中での微生物遺伝子の発現解析。

最近の業績(2005年以降)

（原著論文）

Suzuki, K., Noguchi, M. T., Shinozaki, Y., Koitabashi, M., Yamashita, Y. S., Yoshida, S., Fujii, T., Kitamoto, H. K. (2014) Purification, characterization, and cloning of the gene for a biodegradable plastic-degrading enzyme from Paraphoma-related fungal strain B47-9. Appl. Microbiol. Biotechnol., 98, (10): 4457-4465
Sakai, Y., Ogawa, N., Shimomura, Y. and Fujii, T. (2014) A 2,4-dichlorophenoxyacetic acid degradation plasmid pM7012 discloses distribution of an unclassified megaplasmid group across bacterial species, Microbiology-SGM, 160:525-536
Watanabe, T., Shinozaki, Y., Yoshida, S., Koitabashi, M., Yamashita, S. Y., T. Fujii, Fukuoka, T. and Kitamoto, H. K. (2014)　Xylose induces the phyllosphere yeast Pseudozyma antarctica to produce a cutinase-like enzyme which efficiently degrades biodegradable plastics, J. Biosci. Bioeng., 117(3), 325-329
Suzuki, K., Sakamoto, H., Shinozaki, Y., Tabata, J., Watanabe, T., Mochizuki, A., Koitabashi, M., Fujii, T. ., Tsushima, S., and Kitamoto K. H. (2013)　Affinity purification and characterization of a biodegradable plastic-degrading enzyme　from a yeast isolated from the larval midgut of a stag beetle, Aegus laevicollis, Appl. Microbiol. Biotechnol. 97(17): 7679-7688
Shinozaki, Y., Morita, T., Cao, X. H., Yoshida, S., Koitabashi, M., Watanabe, T., Suzuki, K., Yamashita, S. Y., Kambe N. T., Fujii, T., and Kitamoto, K. H. (2013) Biodegradable plastic-degrading enzyme from　Pseudozyma antarctica: Cloning, sequencing, and characterization. Appl. Microbiol. Biotechnol. 97:2951-2959
Saito, A., Ebise, H., Orihara, Y., Murakami, S., Sano, Y., Sugiyama, Y., Ando, A.,Fujii, T. , and Miyashita, K. (2013) Enzymatic and genetic characterization of the DasD protein possessing N-acetyl-β-D-glucosaminidase activity in Streptomyces coelicolor A3(2), FEMS Microbiol. Lett. 340: 33-40
Nazari, B., Kobayashi, M., Saito, A., Hassaninasab, A., Miyashita, K., and Fujii, T. 　(2013) Chitin-induced gene expression involved in secondary metabolic pathways in Streptomyces coelicolor A3(2) grown in soil. Appl. Environ. Microbiol. 79(2): 707-713
Wang, Y., Nagaoka, K., Hayatsu M., Sakai Y., Tago K., Asakawa S., and Fujii, T. (2012) A novel method for RNA extraction from Andosols using casein and its application to amoA gene expression study in soil, Appl. Microbiol. Biotechnol. 96(3): 793-802
Iino, T., Wang, Y., Miyauchi, K., Kasai, D., Masai, E., Fujii, T., Ogawa, N., and Fukuda M. (2012) Specific gene responses of Rhodococcus jostii RHA1 during growth in soil. Appl. Environ. Microbiol., 78(19): 6954-6962
Wang, Y., Hayatsu, M. and Fujii, T. (2012) Extraction of Bacterial RNA from Soil: Challenges and Solutions. Microbes. Environ., 27: 111-121
Wang, Y., Morimoto, S., Ogawa, N., and Fujii, T. (2011) A survey of the cellular responses in Pseudomonas putida KT2440 growing in sterilized soil by microarray analysis. FEMS Microbiol Ecol., 78: 220-232
Yamamoto-Tamura, K., Ohno M., Fujii T., Kataoka, S., Numata, S., Nakajima, M., Hasebe A. and Akutsu K. (2011) Assessment of the effects of genetically modified Pseudomonas spp. expressing chitinase on the soil microbial community in the cucumber rhizosphere. JARQ-Jpn. Agr. Res. Q., 45(4):377-383.
Nazari, B., Kobayashi, M., Miyashita, K., Saito, A., Wang, Y. and Fujii, T. (2011) High expression levels of chitinase genes in Streptomyces coelicolor A3(2) grown in soil. FEMS Microbiol. Ecol., 77: 623-635
Wang, Y., and Fujii, T. (2011) Evaluation of Methods for Determining Humic Acids in the Nucleic Acids Samples for Molecular Biological Analysis. Biosci. Biotechnol. Biochem., 75(2): 355-357
Ohno, M., Kataoka, S., Numata, S., Yamamoto-Tamura, K., Fujii, T., Nakajima, M., Katsumi Akutsu, K. and Hasebe, A. (2011) Biological control of Rhizoctonia damping-off of cucumber by a transformed Pseudomonas putida strain expressing a chitinase from a marine bacterium. JARQ, 45(1): 91-98
Chu, H., Morimoto, S., Fujii, T., Yagi, K., and Nishimura, S.　(2009) Soil ammonia-oxidizing bacterial communities in paddy rice fields as affected by upland conversion history. Soil Sci. Soc. Am. J. 73(6): 2026-2031
Wang, Y., Morimoto, S., Ogawa, N., Ohmori, T., and Fujii, T. (2009) An improved method to extract RNA from soil with efficient removal of humic acids. J. Appl. Microbiol. 107:1168-1177
Morimoto, S. and Fujii, T. (2009) A new approach to retrieve full lengths of functional genes from soil by PCR-DGGE and metagenome walking. Appl. Microbiol.Biotechnol. 83(2): 389-396
Yoshida, S., Ogawa, N., Fujii, T., and T., Seiya (2009) Enhanced biofilm formation and 3-chlorobenzoate degrading activity by the bacterial consortium of Burkholderia sp. NK8 and Pseudomonas aeruginosa PAO1. J. Appl. Microbiol. 106:790-800
Morimoto, S., Ogawa, N., Hasebe, A., and Fujii, T. (2008) Isolation of Effective 3-Chlorobenzoate- Degraders in Soil using Community Analyses by PCR-DGGE. Microbes. Environ., 23(4):285-292
Saito, A., Fujii, T., Shinya, T., Shibuya, N., Ando, A., and Miyashita. K. (2008) The msiK gene, encoding the ATP-hydrolyzing component of N,N’-diacetylchitobiose ABC transporters, is essential for induction of chitinase production in Streptomyces coelicolor A3(2) Microbiology-SGM154:3358-3365
Chu, H., Fujii, T., Morimoto, S., Lin, X., and Yagi., K. (2008) Population size and specific nitrification potential of soil ammonia-oxidizing bacteria under long-term fertilizer management Soil Biol. Biochem., 40(7):1960-1963
Wang, Y., Shimodaira, J., Miyasaka, T., Morimoto, S., Ohmori, T., Ogawa, N., Fukuda M., and Fujii, T. (2008) Detection of bphAa gene expression of Rhodococcus sp. strain RHA1 in soil using a new method for RNA preparation from soil. Biosci. Biotechnol. Biochem., 72: 694-701
Chu, H, Lin, X., Fujii, T., Morimoto. S., Yagi, K., Hu, J., Zhang, J. (2007) Soil microbial biomass, dehydrogenase activity, bacterial community structure in response to long-term fertilizer management. Soil Biol. Biochem., 39: 2971-2976
Sakai, Y., Ogawa, N., Fujii, T., Sugahara, K., Miyashita, K., and Hasebe, A. (2007) 2,4-Dichrolophenoxyacetic acid-degrading genes from bacteria isolated from soil in Japan: Spread of Burkholderia cepacia RASK-type degrading genes harbored on Large Plasmids. Microbes. Environ., 22: 145-156
Saito, A., Shinya, T., Miyamoto, K., Yokoyama, T., Kaku, H., Minami, E., Shibuya, N., Tsujibo, H., Nagata, Y., Ando, A., Fujii, T. and Miyashita. K., (2007) The dasABC gene cluster adjacent 1 to dasR encodes a novel ABC transporter for the uptake of N,N'-diacetylchitobiose in Streptomyces coelicolor A3(2). Appl. Environ. Microbiol. 73: 3000-3008.
Chu, H., Fujii, T., Morimoto, S., Lin, X., Yagi, K., Hu, J. and Zhang, J. (2007) Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil. Appl. Environ. Microbiol. 73: 485-491
Chu, H., Zhu, J., Lin, X., Yin, R., Xie, Z., Cao, Z. and Fujii, T. (2007) Short-term decomposition of ¹⁴C-labelled glucose in a fluvo-aquic soil as affected by lanthanum amendment Biol. Fertil. Soils, 43: 811-814
Kawase, T., Yokokawa, S., Saito, A., Fujii, T., Nikaidou, N., Miyashita, K. and Watanabe, T. (2006) Comparison of the enzymatic and antifunbal properties between family18 and 19 chitinase from S. coelicolor A3(2). Biosci. Biotechnol. Biochem., 70: 988-998.
Saito, A., Kaku, H., Minami, E., Fujii, T., Ando, A., Nagata, Y., Schrempf, H. and Miyashitra, K. (2006) An enzyme-linked immunosorbent assay (ELISA) to determine the specificity of the sugar-binding protein NgcE, a component of the ABC transporter for N-acetylglucosamine in Streptomyces olivaceoviridis. Biosci. Biotechnol. Biochem. 70: 237-242
Morimoto. S., Togami, K., Ogawa, N., Hasebe, A. and Fujii, T. (2005) Analysis of a Bacterial Community in 3-Chlorobenzoate-Contaminated Soil by PCR-DGGE Targeting 16S rDNA and the Benzoate 1,2-Dioxygenase Gene (benA ).Microbes. Environ. 20: 151-159.
Fujii, T., Miyashita, K., Ohtomo, R. and Saito, A. (2005) DNA-Binding Protein Involved in the Regulation of Chitinase Production in Streptomyces lividans. Biosci. Biotechnol. Biochem. 69: 790-799
Lang, G, Ogawa, N., Tanaka, Y, Fujii, T., Fulthope, R. R., Fukuda, M., and Miyashita, K. (2005) Two kinds of chlorochatechol 1,2-dioxygenase from 2,4-dichlorophenoxyacetate- degrading Sphingomonas sp. strain TFD44. Biochem. Biophysi. Res. Comm.. 332: 941-948

（著書）

藤井毅（2011）環境生物の情報化学物質と機能利用，In農業環境研究叢書第18号　「農業環境研究2001-2010」，pp.137-154，独立行政法人農業環境技術研究所
Morimoto, S. and Fujii, T. (2011) Application of PCR-DGGE and metagenome walking to retrieve full-lengths functional genes from soil. In Molecular Microbial Ecology II, Ed. Bruijn, F. J. de, p.117-123, Wiley-Blackwell, Hoboken, New Jersey
藤井毅（2010）農耕地土壌の生物学的特性解明への挑戦、In メタゲノム解析技術の最前線、p.200-208、シーエムシー出版、東京

（総説）

藤井毅（2011）農耕地土壌におけるメタゲノム解析研究の動向，化学と生物，49 (10): 661-667
森本晶・藤井毅（2011）メタゲノムからの新規遺伝子探索～PCRを用いた様々なアプローチ～，土と微生物，65(1): 66-72
藤井毅（2009）「土壌の微生物分析の基本原理」とメタゲノム研究（共著），「過去」と対話してみませんか？，p.95-100，博友社
藤井毅・宮坂知芳・王勇・森本　晶・小川　直人（2008）土壌抽出RNAを用いた土壌微生物の遺伝子発現解析，植物防疫，62(11)：607-610
藤井毅（2005）土壌DNAの利用　新しい研究手法（その１）特定機能を持つ遺伝子の単離，土壌肥料学雑誌，77巻，115～118
藤井毅・森本晶・長谷部亮（2005）有用組換え微生物におけるスイサイドシステムの発現制御，In 遺伝子組換え体の産業利用における安全性確保総合研究，農林水産省研究成果，428：106～111