These sequence data, the derived SNPs and In/Dels, and additional data will be installed into the previously reported DaizuBase. DaizuBase includes “Unified map”, which indicates the relationship between the linkage map and the physical map, and “Gbrowse”, which exhibits aligned Enrei-BAC clones on the Glyma 1 assembly, and facilitates comparative genome analyses. It will show ‘Enrei’ draft genome sequence data. Next, we attempted to develop a method for comparative analysis among domestic soybeans. The fragmented DNAs prepared from domestic soybean were hybridized with synthetic oligonucleotides designed from end-sequences of mapped Enrei-BAC clones by the NimbleGen sequence capture method. Hybridized DNA was recovered and sequenced with Roche/454 GS-FLX Titanium. To validate this method, sequenced data were assembled and mapped by Blast searches of DaizuBase. Captured sequences corresponded well to the original BAC sequences (Fig. 1), suggesting that this method is suitable for genome-wide analysis of target regions in domestic soybeans.

• Soybean genome sequencing
• Soybean genome databse and resources
• Identification of important soybean genes and development of new strategies for effective breeding